What new can be added in the field of historical studies to implement more realistic programs?

What new can be added in the field of historical studies to implement more realistic programs in dealing with the events of history and interpreting them with scientific sincerity and competence?

26 January 2021 559 1 View

Can any one give me an ideas for my masters thesis (about sustainable energy)?

now i'm in my last master year and i have to choose a topic for my thesis.

18 July 2020 7,750 2 View

How to simulate Lora network applications in NS3?

I need to simulate LoRa network applications in ns3 , in fact, i'm also looking for some examples of moduls that can provide the topology of lorawan network in ns3. Thank you,

23 June 2020 6,220 1 View

What are the tools and methods for analysing the performance of the mechanisms and methods proposed in the iterature for IoT security ?

What are the tools and methods for analysing the performance of the mechanisms and methods proposed in the iterature IoT security ?

20 May 2020 8,421 3 View

Can aliphatic diazonium salts be prepared under special conditions?

16 May 2020 6,466 5 View

What sort of measures should be followed in buildings to prevent pathogenic spread, particularly, in case of coronavirus and similar deadly influenza?

Referring to research papers and general advice are welcomed as part of the discussion

09 March 2020 6,241 3 View

Does the "Schumann Resonance" has any effect on the enhancement or reduction of human brain waves "Especially the alpha wave"?

Is there any article or project about interaction of the "Schumann Resonance" on the brain alpha or theta waves? The Schumann resonances (SR) are a set of spectrum peaks in the extremely low...

02 March 2020 3,146 2 View

How many years does the new compound need to be prepared to be used as an approved drug for patients?

How many years does the new compound need to be prepared to be used as an approved drug for patients

23 December 2019 3,061 3 View

How to double enter 10% of the already entered data?

We plan to enter 10% of the data for a second time to gauge the error mistakes that could have happened. The data is already randomized and picked, but how should I enter the IDs that will be...

23 September 2019 1,681 3 View

Clarification on multiple regression analysis?

Assuming I compute a variable (G) by combining 6 variables (A,B,C,D,E,F). Can i run a multiple regression model with G as the dependent variable and A,B,C,D,E,F as independent variables to...

17 September 2019 8,326 3 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View

How to eliminate Steady state error from Reinforcement learning genetic algorithm code?

The following code (see 1st 2 images attached) is used to produce PID controller values that are designed to control the system (G). The code finds the PID controller values (noted as k) by using...

28 February 2021 6,560 14 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View