Is there any standardized method for multicasting in Multiprotocol Label Switching (MPLS)?

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Comparison of tissue to serum MS data?

Hello, So I have mouse brain tissue MS data from one of my predecessors and I have human serum data from the hospital that I collaborate with. We are trying to find markers for the diagnosis of...

11 July 2024 8,484 0 View

Does anyone know any C++ implementation of Kolmogorov-Arnold network other than mine?

Mine is sitting here: http://openkan.org/DLpiecewiseCPP.html I wish to see someone else's.

06 July 2024 9,974 1 View

Music Therapy used in several colleges.Which one that are great?

I'd trying the several countries with several religions on young and older. what are which books so far. Sound Medicine, Music Medicine on many religions working now.

18 June 2024 1,907 1 View

What is the sampling rate of the Polar H10?

I have read a few papers that have indicated that the sampling rate of the Polar h10 is 1000 Hz. 1) Navalta et al., Heart rate processing algorithms and exercise duration on reliability and...

10 June 2024 4,744 1 View

How come I cannot visualize linear DNA, open circular DNA, and Supercoiled DNA on my DNA nicking assay?

I have already tried different combinations of optimized DNA Nicking Assay protocols. The plasmid DNA that I use is puc19 from a transformed E.coli. Every single time I perform my Assay, I always...

30 May 2024 1,457 2 View

Culturing muscle cells in DMEM?

Hello everyone, I would like to culture muscle cells, specially Human Umbilical Aortic Smooth Muscle Cells (HUASMCs) in DMEM. There are various papers out there where this has been done, however,...

28 May 2024 1,398 0 View

What are the drivers of wetland degradation specifically in western Uganda and how does it affect the livelihood of peeople?

wetland degradation; over exploitation of wetland lands /swamps. Drivers; causes/factors leading to

25 May 2024 3,382 1 View

What method is best for calculating confidence intervals for sensitivity and specificity?

I've used the basic formula : 95% confidence interval = sensitivity +/− 1.96 (SE sensitivity) Where SE sensitivity = square root [sensitivity – (1-sensitivity)]/n sensitivity) 95% confidence...

21 May 2024 2,227 1 View

Issue with DAPI staining on brain (dark spot in middle)?

Hi all, I have a question regarding something odd I'm seeing with DAPI on tissue. I'm trying to isolate the issue to prevent it in the future. I have sagittal brain sections on slides. For some...

15 May 2024 9,194 2 View

Housekeeping protein for serum?

As the title states, I am looking for a housekeeping protein for serum (for Western blots). This research is in the context of pregnancy so it has to take into account normal vs diseased (e.g....

15 May 2024 9,219 0 View

How to increase protein lysate concentration?

I am trying to prepare samples for IP. The pellet size is good and I am using 1% Tx-100 and using a syringe to further lyse the cells and maintain protein-protein interactions. I keep having a...

01 August 2024 7,199 1 View

How do we pick data for determination of Validation Acceptance Criteria?

Hello, colleagues! There is commenting open for new upcoming edition of USP 1033. Validation target acceptance criteria is now different from what it used to be and it doesn't include Cpm....

23 July 2024 7,292 3 View

Is Polyethylene terephtahalate (PET) back support is suitable for electrospun nanofibers for forward osmosis membrane preparetion?

Recently, I am preparing novel electrospun nanofibers for forward osmosis membrane preparation, I am facing following problems: 1. While testing water flux, there is little leakage, although the...

28 May 2024 8,510 2 View

How can I teach children IP?

I am interested in materials that can help teach and guide kids on IP

11 May 2024 6,367 7 View

How to conceive a feasible TDMA schedule to replace/improve CSMA/CA in MANETs ?

feasible: all to one (UDP unicast or multicast) stream traffic, images from all mobiles/nodes sent to one mobile/node. TCP modified for node-to-neighbour operation or end-to-end. Can rely on...

05 May 2024 2,535 1 View

Why does my protein of interest get pulldown during IP, but not its complex memebers?

Hello, I am trying to conduct an IP between two proteins (protein A- 97kd and protein B-37kD). For my experiment, I am using 0.5X RIPA buffer with the addition of protease and phosphatase...

08 April 2024 1,432 0 View

How to prevent detection HA-tagged protein after IP if I look for other proteins?

Hello, I do IP using HA-tagged beads. One of my proteins through which I precipitate complex has HA-tag. I see this protein (with HA-tag) after IP. The signal is strong and bright. The complex...

28 March 2024 4,493 2 View

IP beads for mass spec. Can i freeze the beads?

I need to submit some IP protein G beads for mass spec analysis. However, the mass spec has decided to be not available. Can I freeze the IP beads at -20C ready for the mass spec? If not, what is...

17 March 2024 7,622 0 View

A-Flag antibody works on WB and for IP but cannot be used for blotting FLAG after IP ?

Hello everyone, I'm quitte at a loss here. I'm using the DYKDDDDK Tag Monoclonal Antibody (FG4R) (Catalog # MA1-91878) from thermofisher and while it works beautifully when I blot against Flag or...

13 March 2024 6,342 5 View

Is it ok to use IP lysis buffer instead of RIPA for Western Blot?

Hi everyone, I transfected HEK cells with a protein that is intracellularly expressed through flow. Then I isolated the protein using RIPA buffer protocol, however, I accident added IP buffer...

15 February 2024 8,609 3 View