Is using NaOAc as reaction buffer along with pNG substrate and Na2CO3 as reaction stopper to liquid culture supernatant enough to measure the activity?
I want to cool Natural Gas from 195 Kelvin to 138 Klevin in a heat exchanger by using liquid nitrogen as the coolant medium. When entering the heat exchanger, the liquid nitrogen will have a...
01 March 2021 6,450 5 View
20 February 2021 8,202 6 View
Hi everyone, I am a first year PhD student working on amyloidosis. I would like some tips and tricks to crack efficiently my current nightmare, i.e. the western blotting of the Amyloid beta and...
11 February 2021 2,513 3 View
Hi everyone We need Fast Green to electroporate plasmid into mice embryos, therefore we simply need it to see some color in the liquid we are electroporating so that electroporation is more...
07 February 2021 1,671 2 View
I want to simulate the tribological properties of solids, liquids, and fluids, please suggest good software.
02 February 2021 3,947 3 View
I have trouble with exciting wavelengths higher than 460 nm while e.g. ex with 405 nm works. Filtercubes are correc, as I used them with my old xenon lamp before... I work on axiovert200M and with...
25 January 2021 5,201 2 View
I have a ethanol-water 50% extract of mango peel. I purified mangiferin from the extract with filtration and precipitation. so i obtained a low concentrated extract which i want to se the IR...
24 January 2021 8,880 7 View
This transition phenomena of Globular to spray mode as voltage , and especially current is raised, is very commonly known to Manual Metal Arc Welding (MMAW) and Gas-Metal Arc Welding (GMAW)...
20 January 2021 7,984 2 View
I was reading a reference (Reitz-A Photographic Study of Flash Boiling Atomization). In his experiment, his boundary condition is delta p and constant velocity. The liquid injection pressure was...
18 January 2021 842 9 View
We have an FTIR instrument which was calibrated in transmission/absorption mode using a set of cells for different ranges of D2O concentration. One of the cells at high range broke and the new one...
16 January 2021 4,791 2 View