Currently I am working on sediment phosphorus fractionations. I found in most of the research articles they used the method proposed by Hieltjes and Lijklema (1980). Is there any standard method?
Hi Arif,
a lot of work on P fractions in sediments is based on the sequential analytical scheme proposed by K. Ruttenberg. Some modifications may be necessary to adapt it to your specific samples. Good luck, Michael
Due to difference in the origin of sediment Phosphorous fractions in various systems we cannot follow one method for various types of sediments. Lack of uniformity between different extraction methods , comparison of results is not possible. The sequential extraction scheme by Golterman (1996) using chelating agents is useful for estimating different phosphorus fractions because the chelating agents in this allow a specific extraction of inorganic phosphorus with less destruction of organic phosphorus .
In order to achieve comparable measurements in the determination of phosphate forms in freshwater sediments, the Standards, Measurements and Testing Programme (formerly BCR) of the European Commission has launched a project the first step of which was to select and evaluate collaboratively existing extraction procedures. After evaluation of four sequential extraction schemes, a modified version of the Williams protocol was selected as the most promising method for achieving comparability and was named SMT extraction scheme. (Please refer the paper by Ruban et al., 1999. J. Environ. Monit., 1, 51–56).
A sequential extraction method (SEDEX) developed by Kathleen C. Ruttenberg is a good method to consider in marine sediments. (Please refer Ruttenberg, 1992. Limnol. Oceanogr., 37(7), 1460-1482)
Glad that you could make a choice. But you have not yet revealed the study region.
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