Other then HPLC which analysis at a lab scale can be used to determine the presence of vitamin B12 in fermented broth (Production medium of vitamin B12 containing microbes)
Check AOAC. I think it was spectrophotometric with Bathophenantroline.
Hello, I am simulating gas flow from a high-pressure (700 bar) to a low-pressure container as shown in the attached figure. The red curve shows the pressure curve obtained from the testing, while...
04 July 2020 2,280 2 View
Hello, I am running a 3D density based transient problem with Spalart-Allmaras turbulence model. After few iterations I am getting Negative Nut in xxx cells. I tried to search the cause, but not...
04 June 2020 3,330 1 View
Hello, I went through ansys fluent dynamic meshing guide. It says remeshing can be done only on tetrahedral mesh. I still tried to implement it on hexahedralmesh. It works well. It is bit...
03 May 2020 2,910 4 View
Hello, I am trying to implement capacitive sensor using Arduinio. It is a simple RC circuit. It works as follows, Concept --> Self capacitance of a single plate capacitor Only send pin and...
27 May 2019 6,388 7 View
Hello, I have carried out 3D symmetrical simulations in Ansys fluent. It is a 3D 20 degree rotated model. I created planes at different locations. Forces on these planes should be multiplied to...
24 March 2019 4,262 2 View
I wanted to check the antimicrobial property of my plant extract. Just to check the same I did disc diffusion assay for the concentrated plant extract using Nutrient Agar medium and E.coli...
28 January 2017 7,234 5 View
I am interested in knowing if there is any method to measure the eigenfrequency of very small object of size 5mm x 5mm x 3mm. Material is an elastomer. The problem associated with this is material...
17 May 2015 8,909 7 View
A crude extract of fungal culture using EtOH was subjected to column and TLC and partially purified compound was obtained. UV vis spectrum of the compound/s has max absorbance at 218nm. The...
11 August 2024 9,801 2 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Hello experts, Does anyone know any free software about retention index prediction ?
08 August 2024 7,403 2 View
I'm working on selecting antibodies against a recombinant protein that has a His-tag. My idea is to first bind the recombinant protein to a HisTRAP column and then use this column for an affinity...
07 August 2024 505 3 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
Are there any suggestions or insights you can provide to help address this problem
06 August 2024 1,443 2 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
Mobile apps can be a powerful tool for enhancing academic performance, how can we use mobile apps for improving academic performance
04 August 2024 9,492 0 View
Why is TLC not a form of partition chromatography and paper chromatography not a form of absorption chromatography?
03 August 2024 7,035 3 View
or we can collaborate
01 August 2024 6,705 2 View