Can anybody suggest a simple but efficient chemical treatment of PET sheets to make the surface extremely hydrophilic, for being coated with a thin layer of agarose for hemoglobin electrophoresis ?
We get in India only Ag/AgCl pH electrodes; probably there is only one manufacturer whose electrodes are supplied with any brand of pH meters. We get a significant variation of readings between...
10 November 2018 10,003 2 View
We know about the amount of impurities in agarose like sulfate content increase elelctroendosmosis. Is it known that different buffers used can influence the phenominon of EEO due to different...
10 November 2018 6,862 2 View
Unlike all buffer components, EDTA is tetravalent and has four pK values. Hence it conducts more electric current producing more heat. Since generations they are there in text books and so no...
01 February 2018 9,874 5 View
What this unit identifies for the ion exchange capacity ca. 0.92 meq. g-1. I am a newcomer in the membrane area. I just started. I highly appreciate your help
08 May 2021 0 0 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Anyone can recommend any books on such topics? Thanks in advance.
02 March 2021 8,457 2 View
(This statement is from wikipedia, BTW). What is so special about metallic bond that is limiting high-P low-T state of a matter (as long as individual atoms exist, not white dwarf of neutron star...
02 March 2021 3,309 2 View
The Lanthanide contraction which is the decrease in ionic radii of the elements in the lanthanide series from atomic number 57, lanthanum, to 71, lutetium, is due to poor shielding of the 4f...
01 March 2021 2,272 4 View
Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...
01 March 2021 8,766 3 View
Is the electropolymerisation of polyaniline coating over stainless steel (SS) via Cyclic voltammetry technique is really adherant? Else how to improve the adhesion of the coating, whether any...
01 March 2021 8,220 2 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
I am aiming to determine the ADC values of a commercial fish feed, but I do not have a steam pelleter or extruder available to me. Ideally, the pellets could be coated with some inert marker....
28 February 2021 7,967 3 View