i want to standardize a plant that potentially use as herbal drug, for macroscopic identity, i should measuring the length of the leaves. so, there any sampling method for standardize the length of the leaf? i mean how much leaves that minimum i should take for the measurement
Dear Nyoman,
I am afraid the length of the leaves is not enough as a species characteristic. Leaves have huge variability, therefore they are not suitable.
Best regards, Bozena
Dear Irwan,
Actually there is no such standard methodology to determine the sample size of the leaf sample required for standardize the leaf length. if You consult the herbarium specimen then You should find the same species from different ecological zone and then standardize the sampling number by measuring the shortest one and the longest one leaf of the plants. but if you collect the fresh specimen then you have to standardize the whole things by observing the specimen present in the same community. for further details to study the shape and size of the leaf You consult the literature like the Lawrance, G. M. or the Staflu - Botanical Latins.
with regards
Sunit
Morphometrics is more complex than length, but an optimal sample size is corresponding to the number of replicates that shows the minimal standard deviation. A plot of standard deviation versus number of replicates is an empirical way for selecting the correct number. Light is a morphogen as well leaf age and sampling should keep these factors under control. a ref for shape analysis is http://www.fc.up.pt/dmat/engmat/2012/seminario/artigos2012/PedroSilva/Plant%20species%20identification%20using%20digital%20morphometrics:%20A%20review.pdf
Neither the length of the leaf nor its area holds good as a species characteristic. If we observe the collections of herbarium specimens from many localities with diverse ecological conditions, variability in the length of the leaves becomes evident. In case of many plants, especially those growing partly under water and partly above it, there is likely to be a great difference in the area of the leaves too.
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