Apart from using in vitro methods, I need an in vivo method that can give me an idea if my anti- diabetic plant extract can act by stimulating the opening and closing of calcium and potassium ion channels respectively.
It depends if you mean freely moving animal or anesthetized animal. electrophysiological methods including field potential recording and blind patch can be used for any tissue apart from the CNS. but if you only want to check the effect of a chemical on a single channel properties, in vitro study may be more useful. Also if you don't want to go with the electrophysiological methods, you could use intrapancreatic administration of K and Ca channel blockers.
Thank you so much. I intend to use live animals so I will appreciate if you talk more on 'intrapancreatic administration'.
Check this:
http://linkinghub.elsevier.com/retrieve/pii/S0016508504012260
Please have a look at the discussion took place via:
1. Virginia D.KappelaMarisa J.S.FredericoaBárbara G.PostalaCamila P.MendesaLuisa H.CazarollibFátima R.M.B.Silvaa. The role of calcium in intracellular pathways of rutin in rat pancreatic islets: potential insulin secretagogue effect; European Journal of Pharmacology;702, (1–3), 2013, 264-268.
2. Prasanna K. Dadi Nicholas C. Vierra David A. Jacobson.Pancreatic β-Cell–specific Ablation of TASK-1 Channels Augments Glucose-stimulated Calcium Entry and Insulin Secretion, Improving Glucose Tolerance; Endocrinology, 155, (10), 2014, 3757–3768.
3. A.J.G. Castro et al.The mechanism of action of ursolic acid as insulin secretagogue and insulinomimetic is mediated by cross-talk between calcium and kinases to regulate glucose balance; / Biochimica et Biophysica Acta 1850 (2015) 51–61.
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