' Essentials of Biochemistry'- authored by U. Satyanarayana and U. Chakrapani

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Who can help me with the soft copy of 'Biochemistry' textbook written by Satyanarayana and Chakrapani?

Textbook of Biochemistry.

11 December 2018 7,950 3 View

How is isotopic technique employed in biochemical research?

Principle of operation, methodology and applications. I will appreciate materials on this topic please. Thank you.

09 October 2018 5,812 0 View

Is there any reliable in vivo method to test the effect of an extract on pancreatic beta- cells calcium and potassium ion channels?

Apart from using in vitro methods, I need an in vivo method that can give me an idea if my anti- diabetic plant extract can act by stimulating the opening and closing of calcium and potassium ion...

01 February 2018 5,357 6 View

'Biotechnology'- A textbook authored by V. Satyanarayana

Please who can help with copy of this textbook in PDF?

01 January 1970 9,652 5 View

New Treatment options for Type 2 Diabetes Mellitus

A new experimental drug- TTP399 that works by activating the enzyme-glucokinase is currently under study. what is the latest regarding this drug?

01 January 1970 928 1 View

Biotechnology: Do the Benefits Outweigh the Downsides?

The major apprehension of genetic engineering is that through recombinant DNA experiments , unique micro-organisms or viruses (either inadvertently, or sometimes deliberately for the purpose of...

01 January 1970 3,530 3 View

I need Industrial Biochemistry textbooks in PDF urgently please

Industrial Biochemistry textbooks

01 January 1970 4,187 7 View

The 'Biochemistry' of permed hair

Is there more to a permed hair than the alteration of the disulphide bonds between adjacent strands of protein in hair?

01 January 1970 8,319 5 View

Predicting Depression Levels Using Social Media Posts

The use of Social Network Sites (SNS) is increasing nowadays especially by the younger generations. The availability of SNS allows users to express their interests, feelings and share daily...

01 January 1970 609 2 View

Primary and Secondary Metabolites

Primary and Secondary metabolites of industrial relevance. let's discuss!

01 January 1970 8,805 4 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Can two EC50 values with different hill slopes be compared?

Hello, I fit a classical dose response curve Y=Bottom + (Top-Bottom)/(1+10^((LogEC-X)*HillSlope)) to my data (not related to inhibitors etc). The model I use is purely qualitative but it fits...

23 February 2021 8,155 5 View

Will SARS-CoV-2 Spike be broken into S1 and S2 by denaturing conditions?

Hello, I am performing a western blot of my SARS-CoV-2 Spike-pseudotyped lentiviral particles. I denature my virus by adding concentrated virus to 2X Laemmli buffer at 1:1, then boiling for...

17 February 2021 4,844 4 View

What is the best temperature to coat an ELISA plate?

Hello, I want to control the temperature of my ELISA plate during incubations to get consistent results. In general, what is the best temperature to coat the plate with antibody? I have been...

16 February 2021 2,377 7 View

Is there a way to tell based on appearance which protein crystal should diffract the best?

I have had unheard of success with protein crystallography lately from a super successful protein expression and purification batch. I have attained a lately reproducible vast amount of crystals...

09 February 2021 5,155 5 View

How to measure the concentration of a labeled protein with no tryptophan or tyrosines?

Hey I'm trying to measure the concentration of a protein I've purified and then labeled with Atto 647 flourescent dye. The protein has no tryptophans or tyrosines (although does have...

04 February 2021 4,482 4 View

How to solve error "Cannot find position restraint file restraint.gro (option -r)." on GROMACS?

Hi, I am about to do energy minimization but the above error occurred, I checked my .mdp file and .top file but could not find the problem. TOP file ; Include forcefield parameters #include...

01 February 2021 780 3 View

Why is Fermented Plant Juice (FPJ) always diluted with water? Could it be diluted with other solvents such as rice water?

I am currently making a research paper with my classmates in Philippine Science High School and we are trying to find references that mixed Fermented Plant Juice with other solvents instead of...

17 January 2021 5,099 4 View

Any stable dye to color acrylamide hydrogel beads?

Hi! We are generating hydrogel beads(50-60um) using microfluidics as single-cell beads. To simply all the experiments (especially the centrifuge), I am looking for a dye. The dye should be stable...

14 January 2021 4,383 8 View

How to phosphorylate specific residues on pymol?

Hi everyone, I am using the pyTMs plug in on Pymol to phosphorylate a particular threonine in my protein. I am having difficulty in selecting only one or two residues - it seems I can only...

05 January 2021 230 3 View