08 May 2020 0 7K Report

Water content from amoxicillin trihydrate was measured by Karl Fischer titration and answer is 12.5%. And then, I checked the potency of Amoxicillin trihydrate with the HPLC by comparing the response factor of 86.9 % purity amoxicillin USP RS. So I would like to know that should I need to subtract water content from sample weight taken.? Because I got two answer. If the subtraction of water content, potency is ~97% but 85% without subtraction of water content.

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