My standard test compound showed repellency against Aedes, Anopheles & Culex. The flight orientation results support the repellency result. But in EAG studies no response was observed in all species. While all antennaes respond well to the puff of glacial acetic acid. What could be the reason of no response in EAG studies? 50, 500, 5000 ppm concentration was used for EAG studies.
How sensitive is your EAG setup? Acetic acid excites a lot of cells, but your repellent (if it acts as an odorant) could be sensed by few cells. Distribution of sensilla on the antenna influences EAG shape and amplitude.
Thank you so much Dr. Marianna Zhukovskaya. Before running the standard repellent in EAG, the response was checked by giving a puff of acetic acid which produce sufficient response. I request you to spare some time to elaborate the phenomenon in details.
Thank you Dr. Andreas Reinecke for your valuable discussion and suggestion.
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