Recalcitrant seed short-mid storage method?

Dear peer scientists, Ultradry storage is not suitable for recalcitrant seeds. However, theoretically, if we can stabilize the structure of the subcellular membrane, as well as glassify the...

25 February 2021 2,442 1 View

Can a Golgi-stained dendritic spines picture processed by Image J be directly used for publication?

24 February 2021 3,348 2 View

Implementation of chance-constrained optimization problem via scenario approach?

23 February 2021 7,826 2 View

No title

23 February 2021 4,014 3 View

Build an orthogonal box in ASE?

Hello, I was wondering the best way to build an orthogonal box filled with a defined crystal structure in ASE. I know it could be easily done in LAMMPS, but I guess it is also doable in ASE. The...

21 February 2021 5,899 4 View

Does anyone have a working Guanidine Hydrochloride purification protocol for synthesized ssRNA?

I am trying to in vitro transcribe some fluorescein labeled ssRNA. And I want to purify the ssRNA from the free NTPs and the enzymes (RNA polymerase and DNase I). Due to the characteristic of the...

18 February 2021 4,392 4 View

How to find a particular aptamer complementary sequence?

I can find several papers that give me a guide for choosing a suitable aptamer sequence 1. But how to find a property aptamer sequence 2(partially complementary to sequence 1)?

09 February 2021 3,511 1 View

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam?

Do anyone have a full MATLAB code for structural topology optimization using Genetic Algorithm? Such as a cantilever beam? Could you provide some MATLAB code to me , a beginner for GA. Thank...

03 February 2021 3,789 5 View

How to do DNA normalization based on qPCR values?

Hi Everyone: I have a question regarding to qPCR. I am trying to do RT-PCR, and I want to have equal amount of input DNA for my RT-PCR, so I normalize my DNA input based on qPCR values of a...

31 January 2021 6,977 4 View

MS/MS protein analysis?

Hi Everyone: I want to quantify the relative amount of a protein in 2 samples. I will first digest the sample with trypsin, and subject the resulting peptides to LC-MS/MS analysis. I will then...

25 January 2021 8,333 3 View

Is it harmful to breed for Cre Homozygosity initially?

Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...

24 February 2021 5,759 3 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What will happen (quality or quantity wise) if we transplant 'Aerobic Rice' in puddled condition?

I want to know about the consequences of aerobic rice (both quality and quantity-wise) when transplanted or sown in puddled condition?

08 February 2021 2,343 6 View

What are the new researchable area under Rice crop under Agronomy related to moisture stress and nanotechnology ?

I had already worked on the moisture stress and lot of works being done in rice regarding stress or drought. I want to know some very recent researchable areas in rice crop regarding moisture...

26 January 2021 5,518 7 View

Helicoverpa armigera eggs are not hatching in lab condition, why?

Dear RG friends, I am providing a complete H. armigera diet for larvae and maintaining the eggs in the growth chamber as well as in-room conditions. But since three months, eggs laid from the...

21 January 2021 5,546 32 View

What is the difference between JCR impact factor and Science Citations index impact factor ?

If a journal Impact factor in SCI But no impact factor as per Journal citation reports . Is SCI valid

14 January 2021 325 5 View

How to address the Irrigation and crop management practices database in SWAT model during simulations?

Dear Colleagues I am using the SWAT model in an irrigated basin where I wanna specify the Irrigation database for major crops (sugarcane, maize, rice, wheat, and cotton) in SWAT model...

03 January 2021 3,923 4 View

Can you advise reference publications/standards on characterizing animal genetics research and development activities, and the outputs it generates?

I am doing semantic analysis of science and grey literature publications in the field of animal genetics and breeding. I have a standard dictionary but I am not sure if it captures the state of...

31 December 2020 4,924 3 View

How ash helps in controlling pest,disease control ,is it good to apply or not ?

we all know that it is our traditional practice which we are performing in any problem in our kitchen garden to farm level is it good to apply or not, how it is good or what are its effects.

24 December 2020 2,699 15 View

How Nickel affect biological methane production ?

Soil fertility, plant nutrition

23 December 2020 7,301 5 View