Thank you, Cecilia. We have generated standard curve using a synthetic construct for both Internal control and pathogen. Most of the runs has efficiency between 90 and 105%. I was hoping to calculate Absolute quantification.
Thank you for the publication referral, will read to get more insight. and attempt to calculate.
Dear Bhanupratap,
I have found attached template online that might be of some use to you. With some tweaking you can change it for your data. As Cecilia Mittelberger said, the 2^-ΔΔCT is used here.
Another way is following something along the lines of what has been done in this paper: Article Simple Absolute Quantification Method Correcting for Quantit...
Thank you, Norman van Rhijn Much appreciated for your advice and the article. Just wondered, could you please check if you meant to attach the template as well. Many Thanks
Dr Bhsnupratap, can you please elaborate on the meaning of 105 %. In general, percent score should not exceed 100.
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