Irrespective of the source of the extracellular vesicles, can anyone suggest any existing protocol for isolating the membranes of the extracellular vesicles?
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
how can I incorporate the dyes to each phase ? do I have to heat them !!
21 June 2024 9,377 1 View
Is the Journal of Pedagogical Research a trusted journal to publish in? I checked in scopus website and scimago and I find it is there with scopus Q2 rank; but I have concerns if it is predator?...
08 June 2024 7,678 2 View
I'm considering research topics for my PhD in TOPSIS methodology. Could you suggest some potential titles or areas of study that would be both relevant and impactful?
26 April 2024 602 2 View
I'm currently working on phonon calculations using Phonopy and VASP DFPT approach with a 2x2x1 supercell. I'm seeking guidance on: K-Points Selection: How should I determine the appropriate...
29 February 2024 3,955 0 View
I'm conducting a time course study on gene expression using RT-q PCR for samples treated with 4 conditions: vehicle, RA agonist, Calcitriol agonist or a combination of both agonists over 6 hours....
26 January 2024 7,921 1 View
What are the solutes used to dilute essential oil for DPPH, TPC, FRAP and Beta/Carotene Bleaching Assay? Can I simply dilute the essential oil using 1 mg/mL in methanol/dH2O for all assay?...
21 November 2023 2,599 10 View
with draw the behavior of battery that meaning IV characteristic
09 November 2023 8,991 1 View
The procedure that I used 2 mg beta carotene in 10 mL chloroform with 200 mg Tween 40 and 20 mg linoleic acid. Next, chloroform removal by rotary evaporator and 50 mL of distilled water is added.
08 November 2023 1,890 2 View
What is the standard/positive control that can be used instead of Butylated hydroxytoluene (BHT) or Butylated hudroanisole (BHA)? Sample: Plants’ essential oils
06 November 2023 2,250 8 View
I am trying to coat my micelles and PLGA based nanoparticles with cell membrane using an Avanti mini extruder according to literature . I use 200nm membrane but my particles end up to be 400nm And...
30 July 2024 3,294 3 View
Does it exist some special technique for cutting ceramic membranes for Cross-sectional FESEM or SEM images?
25 July 2024 5,482 2 View
Hello, I am currently working with Malaria Pf/Pan nitrocellulose membranes coated for malaria detection. After drying the coated membranes in an Incubator (overnight at 37°C), I've noticed that...
16 July 2024 7,937 1 View
we have a nafion 117 membrane and we want to know if it has the properties claimed by the sellers. what are the tests available ?
13 July 2024 503 1 View
NA
11 July 2024 8,969 1 View
Is it appropriate to place the ATG codon in front of the gene of interest, since there is a secretion signal that has its own ATG in front of this gene? I need my protein of interest to be...
10 July 2024 3,050 5 View
I have this problem with my western blot for the ABCG2 antibody on nitrocellulose membrane. Help please
02 July 2024 8,715 2 View
I am trying to insert cholesterol inside the DPPC membrane by using gmx insert_molecules -replace. But number of DPPC molecules replaced is much higher than the number of cholesterol inserted?...
30 June 2024 4,160 2 View
Is there an established protocol to measure the permeability of bacterial membranes? Thank you
30 June 2024 6,614 3 View
Several times by placing a restriction on the upper part of the membrane with the aim of preventing the drug from being added from this side, we tried to keep the drug at the top of the leaf, but...
29 June 2024 9,688 1 View