We are currently working on antifungal activity natural products on Aspregillus spp, and Penicillium spp. In our agar well diffusion assay, we observed that in one of the agents, there was inhibition of spore formation not the mycelium of the tested organisms. However, we unable to find any literature to guide as how to report such finds and also any protocol to confirm the inhibition of spore formation. We only have protocol for spore germination inhibition. So would you please share your experience or any literature to help us.
Hi, You could grow the fungus on the agar that have the anti-fungal along with control just the agar medium with out anti-fungal.
Do dilution test to count the spore in both treatment by using the hemocytomete chamber.
Do stock of spore suspension and count spores/ml. Make serial of dilution of your compound to be tested and equal spores in each and take certain volume spread over agar plate and leave in incubator and count. If there is no colony appear; this mean that you got 100% spores inhibition
Hi,
Incubation in unsuitable temperature degrees may delay, change or inhibit spore formation of Aspergillus.
Hi,
Incubation in unsuitable temperature degrees may delay, change or inhibit spore formation of Aspergillus.
Negero: you should check if the inhibition is by compounds produced by the agent or the agent directly. The compounds can be diffusible in liquid medium or they can be gases. Then by gas or liquid chromatography you can identify the compounds. I leave you some paper where you can see the protocols. Luck
Qin Xiaojie, Xiao Hongmei, Cheng Xu, Zhou Hailian, Si Linyuan. 2017. Hanseniaspora uvarum prolongs shelf life of strawberry via volatile production. Food Microbiology 63, 205, 2012
https://onlinelibrary.wiley.com/doi/epdf/10.1111/j.1365-3059.2006.01348.x
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