We can estimate the blood glucose ,starch content of plants,like that is there any methods by which we can estimate the glucose content of bacteria?Actually I want to know about the mode of action of a plant extract which have antimicrobial activity,so by estimating the sugar content I can screen either the plant extract obstruct the carbohydrate metabolism or not.
I f you want to see intracellllular glucose level then fist go for sonication of cell mass then DNS test.
I SUGGEST BREAKDOWN CELL STRUCTURE BY CENTRIFUGATION/SONICATION ETC FOLLOWED BY SUGAR REAGENT TEST LIKE OSAZONE TEST AND UNDER U.V ANALYSIS STUDY ITS QUANTITY BY COMPARING WITH STANDARDS
Take dry wet /live count as toolfor bacterial number/density study
@Kettner what sort of enzyme cocktails are you suggesting..? One more question added to the topic, will this technique be helpful in estimating the glucose content associated with structural components as well..? Or do you suggest sonicating until the final leg where all structural components are neutralized.
I was meaning other organelles, mitochondria, microsomes.. We do have glucose concentrates in these organelles as well. So my question is - should we sonicate to the level of shredding these organelles as well to estimate the level of glucose via the enzyme cocktails which you mentioned? Or would it estimate only the level of free blood glucose.
I am unclear whether you are interested in free glucose (for which the enzyme assay is a good option) or free + conjugated glucose, in which case a monosaccharides analysis by GC might be a better option for you. The Complex Carbohydrate Research Center in Athens GA does such analyses on a fee for service basis and they may be able to help you in planning your experiments.
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