What is the detailed enzymatic reaction protocol for glutathione-dependent formaldehyde detoxification using both enzymes S-formylglutathione hydrolase [EC3.1.2.12] and S-(hydroxymethyl)-glutathione dehydrogenase [1.1.1.284]?
After COVID-19 it has seen that EFL learners technological affiliation has raised. In addition, in the post-COVID period learners started to engage AI technologies like ChatGPT while learning...
08 August 2024 8,964 4 View
How we can cite the papers from ResearchGate. I am trying to create citations for this article, Quantum Machine Learning Algorithms for Optimization Problems: Theory, Implementation, and...
08 August 2024 6,690 3 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
I want to refine one XRD peak of my in-situ xrd but the background is never working good which ultimately fails the refinement. How to refine and adjust the background using GSAS-II
05 August 2024 5,291 2 View
Hi, i would like to simulate an absorption process in Aspen Plus. I want to use the NRTL model und would like to add some individual Henry coefficients. Is that possible and how?
05 August 2024 2,333 2 View
Hello everyone, I'm encountering an issue with my electrochemical impedance spectroscopy (EIS) measurements and would appreciate some insights. Experimental Setup: Electrodes: Gold interdigitated...
05 August 2024 3,783 2 View
AI tools like ChatGPT can enhance research work significantly when used responsibly and in conjunction with thorough human oversight.
05 August 2024 1,842 3 View
Have you ever seen a LC-MS/MS method uses both internal standards and external standards (in matrix matching purpose) but the concentrations of internal standards are outside the calibration curve...
05 August 2024 3,084 6 View
Hi everyone, I am working on brain slices for visualizing a protein in the soma and dendrites, using a fluorescence tag. However, I need a tool (not paid) for reconstruction of the whole neuron,...
04 August 2024 4,725 2 View
Citi BLOC Standard Basket Definitions: A standardized unit representing a fixed basket of construction materials, labor, and equipment costs priced in various cities. Purpose: To create a common...
04 August 2024 8,997 1 View
My study uses C57BL/6J mice and I am required to store portions of the important organs in neutral buffered formalin (NBF). Could I potentially keep those tissues in the formalin for a few weeks...
06 July 2024 8,360 2 View
When I stained the presynaptic sites of NMJs in the mouse lumbrical muscle, I observed significant background in my IFI. I fixed them with 0.5% formaldehyde overnight at room temperature....
16 June 2024 3,428 0 View
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11 May 2024 7,466 2 View
Normally unsaturated polyester or more in general radicalic bonding pastes doesn't adhere to marine plywood, due to the inhibition effect of formaldehyde and tannins. Crestomer 1152 of Scott...
22 April 2024 1,988 0 View
Hi fellow researchers, I have got a noob query regarding the question of the post. I am trying to build a data set of enzymes (from UniProt data base) that would contain protein sequences and...
21 April 2024 7,281 4 View
Polyacetal homopolymer is produced from formaldehyde in industrial scale. The monomer is in gasous state and it needs a tedious purification process. Trioxane is an alternative monomer for...
15 April 2024 8,622 0 View
Hi all, I am a graduate student at ISU working on the nervous/neuroendocrine system of various insects. While still succeful on my slide mounts, I am struggling with the best way to remove fat...
10 April 2024 8,607 0 View
I am doing a reaction in which terminal alkene was broken down into ketone, aldehyde, and another by product aldehyde (formaldehyde). The problem is that this formaldehyde reacts with another...
31 March 2024 7,080 2 View
Can I use feces preserved in formaldehyde to perform the McMaster technique for Haemonchus research?
20 March 2024 1,339 0 View
I tried using oil red O and Sudan black B but I didn't get good results. I used cryostat sections and fixed the tissue into formaldehyde and then sucrose in advance but the tissue field is always...
06 March 2024 6,321 2 View