Methodological confusions regarding deductive and inductive reasoning, hypothesis and theory test?

Hi Colleagues- What do you think about the following methodological issues: 1. Is it methodologically wrong to formulate quantitative research questionnaire based on inductive reasoning? 2. Is it...

21 February 2021 4,634 6 View

Are there alternatives to the Devarda alloy when estimating nitrogen?

Are there alternatives to the Devarda alloy when estimating nitrogen? devarda alloy is reducing the nitrate to ammonium

15 February 2021 8,838 3 View

Up to what extent website personalization can help in creating urge to buy impulsively?

Up to what extent website personalization can help in creating urge to buy impulsively? The digital transformation is making most of the organizations to go online and start doing business through...

09 February 2021 4,430 6 View

Observation instrument for classroom activity in consecutive interpreting?

Hello everybody My research involves an intervention where an instructor delivers a training course to students. My role as a researcher is to write down observations. Do you have any idea about...

03 February 2021 6,853 3 View

Can anyone help to convert ODK XLS into XML?

We want to conduct a verbal autopsy using WHO adult VA updated tools in ODK. The current version of WHO adult verbal autopsy tools is v1.5.3. I have successfully converted VA ODK-XLS v1.4 into XML...

03 February 2021 844 2 View

What is the best way to encode categorical variables in datasets with many features and avoid increasing dimensionality of the dataset?

I am working with a large dataset that has many categorical variables with many classes in each variable. I want to encode the categorical variables without increasing the dimensionality of the...

31 January 2021 6,986 3 View

May I know how to determine the acceptable intakes for new types of nitrosamine discovered in drug?

Currently only common nitrosaminse like NDMA and NDEA have mentioned the acceptable daily intake. Based on EMA guidance, should refer to ICH M7 chapter 7.5 guideline. I have read but could not...

20 January 2021 522 1 View

What are the thermal properties of fibrous, callus and immature bone?

Hi... I am currently working on a project about dental implants. In this project, I require the thermal properties of fibrous tissue, callus tissue, and immature bone. I'd appreciate it if anyone...

20 December 2020 9,043 1 View

What is the best way to define and decide sample size in the case of cross cultural studies?

What is the best way to define and decide sample size in the case of cross cultural studies? I want to decide the number of respondent in two countries which I am considering for my study. One of...

09 December 2020 1,661 11 View

Which MATLAB/PYTHON solver is efficient to solve nondifferentiable nonlinear subproblem?

02 December 2020 2,381 3 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How can you bring two structures together to create an adhesive bond in LAMMPS?

I have created an Ice 1h crystalline structure and an Aluminium substrate structure and equilibrated both at 250K. Now I need to bring them together in a way that an adhesive bond is created....

01 March 2021 3,325 2 View

Necessary tube lenght to cool Natural gas in a dry-ice cooling bath?

I have a tank which contains natural gas at 250 Bar and 293 Kelvin. I want to cool the gas in a dry-ice cooling bath to about 195 Kelvin. The gas will flow in a copper-tube through the bath with a...

27 February 2021 8,128 1 View

Dealing with BMDM seeding and RNA extraction ?

biomedical master student here , i am dealing with mice BMDM and would like some help , i seed 0.5 x 10^6 cells/per well in a 6 well plate but when i extract the RNA i get a very low yeild , i...

15 February 2021 1,102 5 View

How to raise temperature in small increments during equilibration in LAMMPS?

All, I am trying to equilibrate an Ice 1h crystalline structure to 250K in LAMMPS. When I tried to do this at 250K directly, the structure lost it's crystalline shape and became disordered post...

14 February 2021 5,747 2 View

How long can agarose embedded tissues be kept?

I am embedding PFA fixed tissues in low melting point agarose prior to sectioning and I am wondering how long can these can be kept whilst maintaining tissue integrity and fluorescent signal?...

14 February 2021 6,688 3 View

What is a suitable method to freeze feeders-free, serum free episomal hiPSC?

Hi everyone, I need your professional advice: I started growing episomal iPSC (commercial cell line). I grow them in StemFlex medium. What would you recommend as the most effective freezing...

07 February 2021 8,591 4 View

Freeze/Thaw of LS174t cells?

Hello World, I have been working with LS174t. Complete Growth Media- 10% FBS/EMEM. ATCC says to freeze with 5% DMSO/CGM. when I defrost my vials, they are not attaching to flask. vials put into...

01 February 2021 8,723 2 View

What is the Melting point criterion in a MD simulation?

I need a quantitative criterion, something other than structural and visual qualitative features of melting in the molecular dynamics simulations.

26 January 2021 9,096 5 View

Should the Ice 1h crystalline structure be lost during equilibration process in LAMMPS?

I have created an Ice 1h structure and am equilibrating it 100K in NVT ensemble in LAMMPS for a timestep of 0.25fs in 3000 steps. The potential used is TIP4P/Ice. Although the simulation runs fine...

26 January 2021 9,603 11 View