I am not an expert in this field, but I am very interested and have researched to find an answer. Could you please review the response below to see if it is correct?

For the molecular identification of mucorals, commonly used specific primers targeting the ITS1 region are highly effective. The primer pair ITS1-30F/ITS1-217R, as reported in the literature, has shown superior performance in terms of taxonomic coverage and read recovery compared to previously published primers [1]. This primer set is particularly useful in characterizing fungal communities within human samples and has been demonstrated to significantly enhance the detection and identification of various fungal taxa, including mucorals [1]. Another study demonstrates the efficacy of species-specific ITS primers for reliable identification of fungal species, which can be adapted for mucorals [2].

References:

[1] Usyk, M., Zolnik, C. P., Patel, H., Levi, M., & Burk, R. (2017). Novel ITS1 Fungal Primers for Characterization of the Mycobiome. mSphere, 2.

[2] Amicucci, A., Zambonelli, A., GIOMARO, G., Potenza, L., & Stocchi, V. (1998). Identification of ectomycorrhizal fungi of the genus Tuber by species‐specific ITS primers. Molecular Ecology, 7.

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Hi Im already using ITS primers for fungal species identification.. but ITS primers are universal primers give same band size for majority of all fungal pathogens we cannot differentiate between different fungal pathogens like aspergillosis and mucormycosis etc

Humaira Farooq

Mucorales-Specific Primers

MU1 (5'-GGA GGT TTC GAT CAT GTC G-3') and MU2 (5'-GAG GAA GGA AGT GAA CCA ATC-3'): These primers are specific for amplifying a portion of the LSU rRNA gene in Mucorales.

Reference

Lévesque, C.A. & de Cock, A.W.A.M. (2004). Molecular phylogeny and taxonomy of the genus Pythium. In: Phytophthora: A Global Perspective, CABI Publishing, Wallingford, UK, pp. 189-210.