How can I increase cDNA concentartion obtained from viral RNA?

I need good quality and quantity of viral cDNA for NGS analysis. Please suggest how can I increase amount of cDNA obtained from viral RNA.

02 February 2021 8,379 3 View

Pcambia 1301 sequencing ?

I got a LB plate of pcambia 1301 binary plasmid from an institute. I performed miniprep to isolate the plasmid. Now I want to sequence this plasmid. What kind of primers and reagents will be...

03 November 2020 6,075 4 View

Can I use gene specific primers to sequence my insert after TA clonning in pcr 2.1 ?

After ligating the insert in TA vector pcr 2.1, transformation and miniprep. I amplify the gene with simple gene specific primers . Now I want to sequence the insert . Which primers would I need...

31 October 2020 7,077 4 View

Why does the concentration of collagen (hydroxyproline) not consistent each extraction?

We have been extracting hydroxyproline from marine animals and the extraction was repeated several times. However, the results were not consistent and sometimes it turned out 0. We had tried the...

28 October 2020 8,164 1 View

How to correct baseline for Lorentzian noise?

Dear all, Could you please suggest a method for removing background noise which has Lorentzian shape? Best regards, Qurat

30 August 2020 6,907 2 View

What are the best topics for research on environmental informatics, immuno informatics and nano informatics now a days alongwith the best methodology?

best research topics and methodologies on pollulatnts which influence genetic diseases and the treatment through nano technology?

09 August 2020 5,326 1 View

What is the IC50 value of Fluorouracil against HT-144 cell line?

I cannot find the IC50 value of 5-FU against HT-144 cancer cells, as determined via cytotoxicity analysis. I shall be thankful if someone help me with relevant literature.

15 June 2020 4,375 3 View

How to perform leakage radiation assessment from X ray tube with survey meter ?

is it possible to do with just a mini ordinary survey meter ? for a non-separable mAs setting type model, it is said to measure the dose (mSv, mGy) instead of the dose rate (mSv/, mGy/hour). then...

24 February 2020 6,982 5 View

What is the reason behind reduced nanoparticle size after drug loading?

Most of the researches I went through suggest increased crystallite size after being loaded with a drug. However in a unique case, I have observed reduced average diameter after drug loading. Can...

18 January 2020 2,044 11 View

Is it possible to produce sludge from biosorption of dye by using agricultural waste? Mine was producing slimy solution upon the biosorption. How ?

Biosorption, Agricultural waste

25 November 2018 9,526 3 View

What is the best way to keep an anaerobic chamber anoxic in the absence of hydrogen?

I'm a graduate student who works with an anaerobe, so I often work in an anaerobic chamber. I've been working on an assay that involves the steady-state of the quinone/ quinol pool. Anoxic...

03 March 2021 2,971 1 View

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature)?

1. What is the impact of having different scales in a survey? and how can we solve this problem before and after data collection (Literature-based reflection)? Thank you for your time and for...

03 March 2021 2,870 3 View

No title

02 March 2021 3,060 3 View

Good sites to recruit survey participants on a grad school student budget?

Any good recommendations for participant recruitment for online surveys on a grad school student budget?

02 March 2021 4,293 4 View

How does one access the Cranet survey data on HRM practices?

Hello, The Cranet website does not provide any lead to the data obtained from their multinational survey on HRM practices. Any help on accessing the data would be most appreciated. Thank...

01 March 2021 9,536 3 View

Is there a recitation score bank system for fair evaluation and assessment of student recitation?

In actual and virtual class, even if there is rubric for recitation, students may still fail to receive fair grades or scores. Others are active in recitation and others are not or others may fail...

26 February 2021 8,387 3 View

Suggestions for occupational stress surveys?

I am currently writing a masters thesis on reasons for occupational stress in the early childhood classroom, and want to include a short survey assessing participants work-related stress levels. I...

24 February 2021 8,678 2 View

Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations?

Dear colleagues, Do you think there is cognitive oversaturation in the current pandemic? What impact can this have? And other manifestations? The question is mainly aimed at adolescents (pupils,...

23 February 2021 7,796 4 View

Must a control group to be in the same semester that a experimental group for a study in education?

Hi everyone, I'm researching about mobile learning in higher education. Due to the small number of the students in the semesters, I cannot have a experimental and control group together. I'm...

22 February 2021 3,653 11 View

Latent profile analysis based on two variables/sources of data?

Hello, for my master project, I would like to conduct a latent profile analysis about self-regulated learning skills. I would like to take two different measures into account when creating the...

21 February 2021 2,326 3 View