I would be very grateful if some one suggest me the method/procedure of measuring transparency of chitosan/polymer blended films using uv-visible spectrophotometer/amy other method.
What Dzmitry said, is the usual method for this purpose and it is usually worked. In addition, before measuring the chitosan spectrum, you can measure the spectrum of an empty cuvette and set this spectrum as a baseline, so what you finally measure is the absolute spectrum of chitosan. Or if your spectrophotometer has differential mode, you can measure the spectra of two materials (or and empty cuvette and chitosan) and compare the two spectra.