What are the most important explanatory assets ?

06 December 2020 5,474 2 View

What are the methods of writing the interpretation ?

06 December 2020 3,267 3 View

Cell culture techniques: how to deactivate a viral infection while preserving cytokine storm markers?

Hello, I am trying to measure the cytokine storm markers following SARS-Cov-2 viral infection of lung cells and macrophages under different conditions. However, finding a safe way to deactivate...

05 November 2020 1,174 4 View

How can I calculate the am ount required to prepare the following buffer?

ammonium sulfate 0.17 M - polyethylene glycol 4000 25.5% (w/v) - glycerol 15% solution How can I calculate the amount required to prepare the following buffer? I want to prepare 1000 microliter

03 October 2020 4,018 5 View

Why some viral proteins are unable to express and purify in pET28a expression and purification system?

Dear All, I am trying to clone and purify codon-optimized viral proteins using pET28a expression system. Even though I have the perfect insert in the recombinant plasmid, there was absolutely no...

24 September 2020 3,635 8 View

Non fluorescent E. coli GFP?

I Have a problem with E. coli GFP, I am culturing them in LB medium/ampicillin + IPTG, but not all the cells fluorescent after UV excitation (fluorescence microscope) although they grew in the...

15 September 2020 4,636 1 View

How are parents guiding their children with learning difficulties?

Parents' educational level is essential in raising the level of education

14 September 2020 6,306 4 View

Is it possible to integrate ratio measures with ordinal survey scale?

24 June 2020 205 1 View

X-ray Experiments for antibody S309 Zero ini on COVID-19 ?

18 May 2020 3,666 6 View

Why encrypt Bloom filter and query in a different way in the following paper in searchable encryption?

I was read in paper in searchable encryption that encrypt bloom filter index in the following way: 1- generate secret key to encrypt bloom filter and query is: the secret key sk (M1, M2, s ),...

03 March 2020 1,448 5 View

What is the production process of CaFe ferroalloys?

I looking for ferro calcium production process

03 March 2021 1,755 4 View

Chlorinated organic formation in odor control scrubbers using hypochlorite?

I'm involved in a study of odor control technologies for municipal wastewater treatment plant. One of the control options involves a chemical 2-stage (acid/alkaline) packed bed scrubber. The...

03 March 2021 3,661 2 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View

How can we theoretically calculate Elemental analysis(percentage) of atoms in covalent organic framework?

For example in one research article the Elemental analysis values of COF-TpPa-1 ( Triformylphloroglucinol (Tp) (63 mg, 0.3 mmol), Paraphenylenediamine (Pa-1) (48 mg, 0.45 mmol) are as follows...

24 February 2021 1,265 6 View

How to Control Whiteflies in coconut leaves?

24 February 2021 1,005 1 View

How we can measure Kemp % in wool without using of benzene?

Would you please let me to know about the methods for measurement of medullation and Kemp % on animal? My problem is that the wool sampling and sending to laboratory is not possible. So I would...

21 February 2021 7,260 4 View

What is the best etching method for Mg-Ca alloy?

I have used Picral etchant (4.2 g Picric acid, 10 mL Acetic acid, 70 mL Ethanol, 10 mL distilled water) but it didn't work. Is there any alternative?

21 February 2021 2,558 5 View

What reaction will occur when adding hexamine into ammonium nitrate in an acidic medium?

Hello, I am investigating properties of water in oil emulsion explosives. And adding hexamine (hexamethylenetetramine) into one of the phases that are used to make the emulsion should increase the...

21 February 2021 8,680 1 View

How to get/count refractive index in gasses?

20 February 2021 8,202 6 View