Questio regarding micro/nano particles fabrication ?

I will start to fabricate micro/nanoparticles for growth factor release and I check out a procedure quite nice, however, it is lacking some details and since I don't have much knowledge doing this...

20 February 2021 199 2 View

How do you prepare a standard dilution for qPCR?

I am very new to quantitative PCR and need some help for this technique. For my experiment, I need to perform a quantitative reverse transcription PCR. I extract the RNA from my pellet and...

05 February 2021 9,834 4 View

Can I modify vermiculite or perlite by adding nutrients to control a certain situation?

I need to assimilate a determining condition for the plant. However, I need to ask you if it is possible to change the properties and concentration of plant nutrients in this material like perlite...

04 February 2021 8,616 11 View

What is the half-life of TNF-alpha in vitro?

I'm designing a low-grade chronic treatment with this cytokine and I'm wondering how regularly to change the culture medium based on the half-life of the protein in an in vitro environment....

24 January 2021 5,988 2 View

Which sampling techniques can be used for huge flying insects?

Tropidacris collaris is a grasshopper. Adults, which can reach more than 15 of length, are strong flyers and actually they resemble small birds or bats. Most of the time T. collaris is found in...

13 January 2021 3,735 5 View

Does freezing treated cell pellet at -80C damages them?

Hello, I hope you are all doing well. I have a question regarding my treated cells group. I plan to do western blot and qRT-PCR for my treated cells. However, to save time, I would like to...

06 December 2020 1,390 5 View

Why do some primers have transcript variants/unintended templates for different genes?

Good day, I hope you are all doing well. I was designing primers for Caspase 9 and sometimes, the designed primers in NCBI have transcript variants for the same gene (Caspase 9) but at the same...

05 October 2020 352 3 View

Do I need to design forward and reverse primers within an exon region for QRT-PCR?

Good day, I hope you are all well. I am new in designing primers for quantitative reverse transcription PCR. I am using NCBI to design my primers and my question is: Do I have to design my...

04 October 2020 7,678 7 View

Why is my molecular rotor displaying a bathochromic shift in toluene compared to MeOH or EtOH?

Hello, I am studying the photophysical properties of a molecular rotor which unexcpectedly, shows a lambda(max, absorption) = 565 nm in toluene, but a lambda (max, absorption) = 540 nm in MeOH...

15 September 2020 7,830 3 View

Animals - Special Issue, "Learning Theory Applied to the Welfare of Animals", looking to submit?

Dear Colleagues, Please see the following link and information for more details. If you are thinking about submitting, please feel free to reach out to me directly here or at...

09 September 2020 6,361 1 View

Is this the fragment of a sauropod tooth?

Dear colleagues. Fragments of this species are very rare from a terrestrial Lower Cretaceous locality from Germany. Up to now from this locality sauropods could not be proved with certainty. Other...

14 February 2021 2,310 1 View

Does someone know which structure could exit the olfactory bulbs at their antero-dorsal top ?

Hi people, I am studying bat brains through endocranial casts and I cannot identify which structure could be present in sort of a canal located dorsally to the cribriform plate. It is like...

27 January 2021 797 6 View

Which is the most recent and updated classification of Class: Insecta?

Based on the many publications from several decades, we came across many classifications of Insects. Among them which one is more updated and approved classification that can be used for research,...

05 January 2021 3,668 9 View

How can we say a species of insects got extinct?

02 January 2021 4,232 10 View

Could anyone provide me any useful website or bibliography to learn about the principles of taxonomy in Biology and Paleontology?

I am an undergraduate and I would like to broaden my knowledge on this subject, beginning by its principles. Maybe it would help that purpose to broaden my knowledge on Phylogenetics as well....

08 June 2020 1,343 2 View

Is it better to use base e (LN) or base 10 (LOG10) when making estimates of body mass from linear measurements?

For linear regressions between linear measurements and body mass, which transformation is better to use? base e logarithm (LN)? or base 10 logaritm (LOG10). Apparently they do not make such a for...

08 April 2020 5,201 12 View

How can I get individual rarefaction on PAST4 (Paleontological Statistics software)?

Hi, I'm currently working with PAST 4.01 and I have my data organized on a table of 4 columns (treatments) and 45 rows (different bacteria genders) with the amount of genders found in every...

31 March 2020 1,914 1 View

Mammary glands in Platypus ?

Dear researchers, Can anyone help me to identify these odd mammary glands in Ornithorynchus anaticus ? I struggle to understand its mecanism : The secretion from all these flat lobes have a...

03 March 2020 8,275 2 View

What kind of fossil algae is this?

Hi everybody. I found this in a thin section of an oncolitic limestone that belong to late Jurassic - early Cretaceous period (Tithonian-Valanginian). im a begginer in this matters but my first...

01 February 2020 6,783 3 View

Can somebody help me with recognizing this fossil plant ?

Please help me in the identification of this well preserved fossil plant found in Tufa . Thank you in advance !

05 November 2019 4,783 6 View