Densitometry of 1D gel electrophoresis
Dear Robert,
Thank you very much, really appreciated it ,
Rana.
Hello Majid. Use Image J. Simple and Best
I have a raw file of tandem mass spectrometry (LC-MS/MS) data. I want do online protein databank search for my protein identification and its homology.
30 January 2021 3,081 3 View
I work on gastric cancer. I want to separate the top layer of tumor cells from the lower layers.
25 January 2021 6,663 1 View
Hi everyone, I want to mesh a part in Abaqus for further use in ANSYS Fluent because this way, it is much easier and faster to get a high-quality mesh. Is it even possible to generate a file...
15 January 2021 5,665 3 View
Dear RG members, Excluding the (intrinsic) factors such as quality and content of an article, what are the extrinsic factors/predicors that influence the citation rate of a research article? Is...
07 January 2021 3,410 3 View
I have modeled the RC beam with simply supported BC and I have got the frequency value for the case of the beam, but I have 150 modes for each analysis and I have to see them one by one to find...
05 January 2021 8,626 3 View
I have prepared a relaxor ferroelectric (KNN - BZZ solid solution) bulk sample. During P - E loop measurements, we experienced saturation electric field of about 100 kV/cm before dielectric break....
29 December 2020 9,080 3 View
What software do you prefer?
29 December 2020 889 3 View
i have multi x ray images after preprocessing on it , i saved them as a vector in file .dat but this file not accepted in next stage (convolution neural network ). how can i save all image in...
27 December 2020 1,369 4 View
It is for my research purpose
21 December 2020 833 3 View
the study of microorganisms is called microbiology Microbiology is the study of microscopic organisms, such as bacteria, viruses, archaea, fungi and protozoa. This discipline includes fundamental...
13 December 2020 8,287 6 View
Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...
03 March 2021 4,275 4 View
Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue
02 March 2021 5,433 5 View
I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...
02 March 2021 9,157 1 View
Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.
01 March 2021 9,952 3 View
Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...
01 March 2021 2,622 3 View
I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...
25 February 2021 8,140 3 View
Hi I am very new to proteomics analysis. I have got raw files from core facility running thermofisher TMT 16plex 4 hours. I cannot find on maxquant the option TMT16Plex there is only up to 11...
25 February 2021 1,675 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...
22 February 2021 1,789 3 View
This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...
22 February 2021 338 9 View