Is there any food which can prevent the blood clot formation in the lungs due to Coronavirus infection?

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Does Anyone have expertise in in vitro transcription and RNA pull down assay?

I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.

07 August 2024 6,622 1 View

How can I make complex file of ligand (including flexible residues from protein) with protein Structure after AutodockFR docking?

I have performed the Molecular Docking using ADFR suite, I have obtained the files in _out.pdbqt format for ligand docked poses, upon inspection, both visually and reading the pdbqt files of...

08 June 2024 8,130 2 View

How does uplink IM-NOMA works?

I am currently studying index modulation aided NOMA technique but my mind blown up with mathematical expressions. Can someone explain to me these; How Index Modulation aided NOMA (IM-NOMA) works?...

27 April 2024 2,736 1 View

Does non terminal his-tag work in purification?

i am working with membrane protein and there is a his tag 4 amino acid above the c terminus (seq HHHHHHDLEY). anti his antibody does not recognise the his tag in western blotting. i was wondering...

14 April 2024 2,675 5 View

How to interpret liquid chromatography mass spectrometry (LC-MS) result?

I have done the LC-MS result of my complex and its molecular weight is supposed to be 515.5 g/mole. I want someone who is experienced in LC-MS data interpretation. I have attached the picture of...

16 February 2024 382 2 View

How to slow down 1165 based lift-off for thermally deposited metals on semiconductors ?

quick summary: I am looking for a way to slow down lift-off (1165 or acetone) for a given material system (changing materials to solve the problem is not possible) This is a relatively general...

08 January 2024 8,955 2 View

How to Report an Authorship Misrepresentation?

Dear ResearchGate Support Team, I hope this message finds you well. I am writing to bring to your attention a concerning matter regarding the authorship of certain articles on ResearchGate. It has...

25 November 2023 3,908 1 View

while getting data that whether newts can be sustainbale in farm land ponds in compare with natural reserve?

how can we calculate average heterozygocities and unique alleles also how we can compare both populations of different ponds?

21 November 2023 3,238 1 View

Any genetics expert? or the person who is doing research on newts?

i need assistance with few terms your help will be appreciated.

21 November 2023 4,398 1 View

What are the hot topics for Research in Machine Learning in the field of Computational Science and Engineering?

Using machine learning algorithms

26 July 2023 3,025 2 View

Use of NC or NTC to descibe negative control?

Hello, I would like to ask which is the best terminology to use for describing a negative control in an experiment (drug testing in-vitro, viral infection): NC (negative control) or NTC? Thank you...

18 July 2024 4,183 2 View

Assistant with TUNEL technique for A549 cells, switch from trypsinized cells to adherent cells?

Hi, Im using the kit from abcam (ab66108) In situ Direct DNA Fragmentation (TUNEL) Assay Kit for A549 cells, the kit required to trypsinize the cells which its okay (in theory) but the problem is...

07 May 2024 4,706 0 View

What are Trends in viral infection among cancer patients?

Hello every one Can any one suggest me trends subjects in viral infection in cancer patients for PHD thesis? Thanks in advance.

05 May 2024 5,263 3 View

What is the difference between chemiluminescence assay and ELISA for viral testing in terms of specificity and sensitivity?

I want to know the which assay is better and its reliability while testing the viral infection.

10 March 2024 7,056 0 View

Freshwater shrimps and affected gills. Is it a parasite?

Freshwater shrimps of the genus Neocaridina which are kept in captivity (aquarium) have "dirty" gills (visible through the transparent carapace). The "parasite" was not motile. Mortality was...

22 February 2024 542 1 View

Why does my sample show inconsistent band pattern in western blot?

Hello, I'm having a bit of trouble doing the Western blot experiment. Even though I load the same sample, the band appears inconsistently (in all the trials). (I usually store the samples at -20°C...

18 February 2024 2,863 1 View

Research Practice Gap. Wearing the Mask When COVID-19+ Detrimental Rebreathing of Virus?

Most research conducted in the past four years on the efficacy of mask-wearing during the COVID-19 pandemic has primarily focused on its role in preventing person-to-person transmission and...

25 January 2024 7,347 0 View

Is the bacterial and fungal infection associated with Covid-19 specifically after infection? Is it a secondary infection or a co-infection?

Question ❓

22 January 2024 1,144 2 View

After qPCR analysis of gene expression, when is difference in relative fold change actually relevant?

During work for my undergrad thesis, I've examined and compared gene expression of stress-induced genes in plants challenged with a fungal infection. I have calculated the relative fold change and...

22 November 2023 5,518 3 View

Why does primary cell culture exhibit poor growth?

Hello, I am trying to perform a primary culture of tumor tissue using DMEM+1 * ps+10% FBS medium. On the 4th day after inoculating tumor tissue blocks from mice on the culture dish, we observed...

13 November 2023 939 0 View