Is there any fluorescent equivalent of giemsa stain/Wright's stain for G-banding in karyotyping?
Theoretically, staining achieved with quinacrine (Q-banding), DAPI, Hoechst should provide the same band pattern, however, have you ever seen it (identical number of bands)? If so, can you provide a paper in which they claim that? Do you know any other fluorescent stain which can achieve banding identical to G-banding?
I would appreciate also a negative answer.
Thank you
In general, trypsin Giemsa-stained and quinacrine Hoechst-stained images are inverted black-and-white stained images. However, the trypsin Giemsa-stained image has higher resolution and the bands are more detailed. Quinacrine Hoechst staining analysis is simple, but it would be difficult to detect structural abnormalities as small as 2 Mb.
The best,
Uno
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