Previous I've extracted polyphenols from the aqueous extract of plant by using di-ion resin. But I want extract polyphenols from other solvents (like n-hexane or ethyl acetate). is there any any effective process of extracting Polyphenols from plant extract.
Beside this what is the suitable procedure for the isolation of proanthrocyanidins from plants?
thank you in advance.
Dear Kushal,
The following recent review article entitled "Techniques for Analysis of Plant Phenolic Compounds " published in Molecules 2013, 18, 2328-2375; doi:10.3390/molecules18022328 along with the other attached publications fully cover the answer to your question:
Abstract: Phenolic compounds are well-known phytochemicals found in all plants. They consist of simple phenols, benzoic and cinnamic acid, coumarins, tannins, lignins, lignans and flavonoids. Substantial developments in research focused on the extraction, identification and quantification of phenolic compounds as medicinal and/or dietary molecules have occurred over the last 25 years. Organic solvent extraction is the main method used to extract phenolics. Chemical procedures are used to detect the presence of total phenolics, while spectrophotometric and chromatographic techniques are utilized to identify and quantify individual phenolic compounds. This review addresses the application of different methodologies utilized in the analysis of phenolic compounds in plant-based products, including recent technical developments in the quantification of phenolics.
To view the full review article, please see attached file.
Hoping this will be helpful,
Rafik
Dear Kushal,
The following recent review article entitled "Techniques for Analysis of Plant Phenolic Compounds " published in Molecules 2013, 18, 2328-2375; doi:10.3390/molecules18022328 along with the other attached publications fully cover the answer to your question:
Abstract: Phenolic compounds are well-known phytochemicals found in all plants. They consist of simple phenols, benzoic and cinnamic acid, coumarins, tannins, lignins, lignans and flavonoids. Substantial developments in research focused on the extraction, identification and quantification of phenolic compounds as medicinal and/or dietary molecules have occurred over the last 25 years. Organic solvent extraction is the main method used to extract phenolics. Chemical procedures are used to detect the presence of total phenolics, while spectrophotometric and chromatographic techniques are utilized to identify and quantify individual phenolic compounds. This review addresses the application of different methodologies utilized in the analysis of phenolic compounds in plant-based products, including recent technical developments in the quantification of phenolics.
To view the full review article, please see attached file.
Hoping this will be helpful,
Rafik
Dear Kushal,
I have sent you other 4 publications attached to my first answer.
Good luck,
Rafik
See Dr. Kushal, it depends on which specific poly phenol you are interested in.
actually not a specific one, I want isolate polyphenols from my ethyl acetate fraction.
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