I have studied cytotoxicity assay using Brine Shrimp Lethality bioassay and calculated the LC50 value using Excel and Graphpad Prism. But got different LC50 values using mentioned tool.
Which one is correct Excel or Graphpad Prism?
It is not about what software you use to calculate your LC or EC50, or for that matter anything. It comes down to the equation you fit your values to. If you are using the same equation to fit your data in both Graphpad and Excel, you will get the same results. These softwares aren't doing some magic, they do exactly what it is being told to do. You can check on graphpad, the equation that is being used to fit the data.
It is not about what software you use to calculate your LC or EC50, or for that matter anything. It comes down to the equation you fit your values to. If you are using the same equation to fit your data in both Graphpad and Excel, you will get the same results. These softwares aren't doing some magic, they do exactly what it is being told to do. You can check on graphpad, the equation that is being used to fit the data.
Obviously Dr. Ahmed's answer is correct. The same mathematic procedure should give the same result regardless of the software.
The observed different results can stem from that Graphpad Prism can use/select different fitting methods and one can apply weighting to make more or less emphasis on outliers and high versus low values.
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