Generally we have performed acute toxicity study and based on that acute toxicity study result we fix the experimental dose of plant extract. Taking consideration the safety of the extract, 1/10th of the maximum dose given in the toxicity study limit test was considered as a middle dose. Double of the middle dose was assigned as high dose and half of the middle dose consider as lower dose.
My question is, may we follow the same procedure during the standard drug dose selection?
If it is a standard positive control, that means somebody should have already done experiments with it and that is why it is already an established standard.
Therefore, you need to screen the literature to find what doses have others used.
If you are using this chemical for the first time (with no literature present), then you need to set the doses yourself on the basis of initial acute study.
A S M Ali Reza , you are right about the dosage levels but you have to optimize the "standard dose" according to your study.
Dr. Numan Fazal , How do i optimize the standard dose with my study? Would you please share any references?
Thank You
Actually, I don't understand the concept of 'standardizing the standard dose'. If it is a "standard positive control" then it means that it should have already been used in various laboratories, and its dose-response curve has already been studied, toxicity effects established and published. So, the dose used across various laboratories should be same. It normally holds true for the standards used in various in vivo and in vitro assays.
I want to add here that there might be some chemical which has been used in one lab (no other reference available) and they got positive results with that, which might not work in your hands. This might be because they would have just manipulated something for sake of publication, or there might be lab to lab variation. In this case, you need to cross-check everything as what could be the cause of variation.
I would like to know the positive control that you want to use, if you can share..
Dr Varun Ahuja
Thanks for your comments.
We have been using plant extract for anxiolytic and antidepressant-like effects in several mice model. Our sample concentrations are 100, 200 and 400 mg/kg. In this case the standard drug Imipramine hydrochloride (10 mg/kg) has been used by previous researcher. My question is how do they select this dose? or What are the reasons for choosing the dose of positive control?
- Badges
- Science topic
- Similar topics
- Chemistry
- Pharmacology
- Pharmaceuticals
- Drugs