looking for a dataset
Hi, I have been working with 3T3-L1 cells for over a year now.However, over the last couple of months they stopped differentiating into adipocytes. I seed at 200k cells/well (12 well plate), and...
23 May 2024 3,356 1 View
What is the optimum concentration to be used for the miRNA mimic and if there is a good working protocol please advice
17 December 2023 5,683 0 View
Hi, I have a PCR product for DDL gene for Enterococcus faecalis with non-specific binding as appears in the gel every time I run a gel for the positive control I see two bands the first is my band...
29 October 2023 1,315 3 View
I use molecular devices setup to patch acute brain slices, and I face this problem of "overloaded"sign that appears on the amplifier, and even when I try to autocomponsate the pipette it shifts to...
09 August 2023 4,032 3 View
Some genes that resulted from my differential gene expression analysis don't have corresponding ENTREZ ID and I want to perform KEGG pathway analysis but most tutorials analyze data with no...
24 July 2023 3,961 2 View
What is the minimum number of animals in each group used in expermint?
09 July 2023 5,390 3 View
I think of applying the drug as a continuous infusion over 36 hours. I thought of putting it on a bio-compatible membrane over the surface of the brain is this possible? has anyone seen a study...
26 April 2023 8,612 0 View
Hi everyone, I have a problem with my PCR and tried so many trials to get my product..I am working on RAG 1 gene in birds...my PCR doesnt work at all..however..I used many recipes and programs to...
27 October 2022 6,814 3 View
I am following the examples from Schrödinger software but I still face difficulties. It would be nice if I can get help here.
19 May 2022 2,607 0 View
Dears, If there any open source (program) to plot the Nanopartciles and attach ligands. From such structure I would like to calculate the DFT? kind regards
23 March 2022 3,510 5 View
Can anyone explain this method? Especially the last statement where it says only at 1.5 to 2.5mins was the MS/MS connected to the UPLC. How is that possible, is it a feature in this specific...
11 August 2024 8,141 3 View
Let's say we have a standard, regular hexagonal honeycomb with a 3-arm primitive unit cell (something like the figure attached; the figure is only representative and not drawn to scale). The...
07 August 2024 1,937 1 View
Hello What should be done to separate and identify organic acids in HPC when their RetTime is the same?Like oxalic acid with Propanoic Acid.or acids that have a very close RetTime.
07 August 2024 8,782 3 View
I am planning to collect human fecal samples for metatranscriptomic analysis using MGI. These samples are from indigenous people living in a region with high temperatures. I will have access to a...
06 August 2024 1,367 3 View
I've seen articles that primarily focus on alpha and beta activity in the frontal regions, but these studies often compare healthy subjects with those having various pathologies. I haven't seen a...
31 July 2024 7,259 1 View
Hi everyone, I'd like to better separate two close peaks coming from a bioconversion sample, I'm currently using a C18 KromaPhase 4,6mm Ø.I. SCHARLAU, particle size (µm): 10, pore size (Å): 100,...
30 July 2024 435 3 View
Our department has recently acquired an HPC (High-Performance Computing) system, and I'm thrilled to take my molecular dynamics calculations to the next level using Desmond. I used to run my...
28 July 2024 6,553 1 View
AGILENT HPLC 1100 SERIES operation assistance needed.
25 July 2024 10,045 2 View
I have designed magnetic material with different biasing conditions in HFSS. Now I want to give an RF AC signal and do a transient simulation in HFSS. Is it possible to do in HFSS? Please help me...
24 July 2024 9,319 5 View
Time-Frequency Domain
19 July 2024 8,031 2 View
