How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

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03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

How to quantitative analize the data of TNBS assay for gelatin?

Estemeed colleagues, I found some issues regarding the quantification of the data for TNBS assay. There are different protocols on how to perform that but it is clear to me the "fil rouge" that...

02 March 2021 2,616 1 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

Why does the testosterone dissolved in dmso and methanol turns into a white dispersed precipitate when subjected to serum free media?

1mg of testosterone is dissolved in 20 microlitre of DMSO and Methanol respectively. The interpretation showed a positive result. However, when 1 ml of serum free media is subjected to the...

27 January 2021 8,213 1 View

Is there a formula or standard protocol for steroid hormones treatment in mice or rats?

hi, I'm going to determine the dosage of testosterone in order to inject to rats or mice subcutaneously. I wonder that is there a particular dose or multiple doses for that or not. thanks.

05 January 2021 5,921 2 View

Writing participant characteristics in method while being LGBTQI+ sensitive?

I apologize in advance if I come across as ignorant. I am trying my best to educate myself on LGBTQI+ community and how to write them in academic writing. I have a sample of X participants in a...

29 December 2020 9,011 3 View

Are there only winners in the world of exism?

BREXISM finally is over and they got a trade deal with europe now, but exism left the UK poorer according to the news Brexit is finally done. It will leave the UK...

23 December 2020 4,071 6 View

Hormone levels in male mice ?

Dear Colleagues, Can anyone tell me if there are Data about hormone levels in male mice , E2 Estrogen, and Testosterone blood levels in male mice. Thank you very much.

20 September 2020 1,035 3 View

What software is used in education?

I have to find out what software and operating systems are used in education. More into lower education than in high and in different countries (USA, Australia, Great Britain, Germany, France,...

27 July 2020 5,762 2 View

Would you be willing to contribute your expertise and knowledge on the following topic below?

To all the project management professionals in the United Kingdom, Thank you for taking the time to read this post. I'm currently a final year master's student at Birmingham City University doing...

02 July 2020 1,245 8 View

Are the morals (sic) of a writer, artist or scientist part of their overall assessment?

Does it matter if Picasso was driven by destructive maschismo in his relationships, Oscar Wilde employed rent boys, Einstein was promiscuous (maybe) when heralding their different achievements?...

07 April 2020 1,410 6 View

GBP loss of value vs euro, WHY? What impact?

What factors brought the euro value of the GBP from 1.5 euro in 2004 to 1.06 euro today? A -41% loss, of the British Pound, that's a lot. How could this happen? Is it a cause a consequence of...

25 March 2020 9,091 18 View

Do you work in primary education in the UK?

If you work in primary education or with primary aged pupils either who have or do not have SEN. Then would you consider filling out my questionnaire as part of my dissertation into professionals...

23 January 2020 5,434 3 View