land and property rights of the tribes of nilgiris before the advent on colonialism
Sure, the land were classified as stool land or skin land, family land and private land. Stool land were dominant since rule of the day were by chiefs, followed by the family land
I am working on transparent/translucent zirconia-based ceramics. In many papers, I have seen that they are reporting both In-line transmission (%) & Total transmission (%) value of...
20 February 2021 9,405 6 View
Hello everyone, I want to perform some gaussian calculations and want to use Materials studio for my research work. Can anyone guide me how to get access to supercomputer resources in India for...
15 February 2021 8,264 3 View
Dear community, I am currently writing my master's dissertation. As a part of the method, I have to include views (interview) of experts for the empirical analysis. The problem is I have not...
31 January 2021 3,438 3 View
Am in Peptide / Protein Research, may i know what useful analytical information we are getting from 13C & 1H NMR spectroscopy analysis? Somebody getting NMR for smaller proteins, any useful...
05 January 2021 1,196 7 View
Suggest some good articles on Genomics of this new COVID strain.
03 January 2021 7,635 13 View
I have checked annealing temperature through gradient PCR. I have checked my primer in NCBI. I have checked PCR reagents concentration. Please suggest me anyone thanks
12 December 2020 1,969 2 View
Please guide me in solving the structure and preparing a CIF file out of the results, I have only powder xrd data which I have attached here. I already have EXPO2014 and fox. Both these software...
06 December 2020 641 8 View
Hello everyone, Please help me to create a CIF with two different molecules which are linked through hydrogen bonding in the same crystal lattice. I am attaching an example to understand what I...
16 November 2020 8,056 3 View
Is it possible to perform gaussian calculations using molecular structure downloaded from chemspider or pubchem? The active pharmaceutical ingredient I am working has complex structure and I am...
29 October 2020 3,942 3 View
In high pressure liquid chromatography (HPLC), the compound is injected through a column of different sized beads.The amount of time it takes for the compound to pass through the column is the...
21 October 2020 8,801 3 View
Hi, I'm looking for data (mainly related to management: growth rate, canopy size, soil and climate preferences, etc.) about tropical trees used in tropical agroforestry. Have you ever heard about...
28 February 2021 7,356 8 View
I have a protein with a 10xHis-MBP tag on the N-terminus. I'm fairly certain I am expressing my protein, but I'm not getting a signal on my western blot. Is there a possibility that the 10xHis tag...
25 February 2021 8,404 3 View
I am facing difficulties in cloning a 1kb gene into a vector (pJIT163). I have my gene on interest (GOI) in pUC57 and want to clone in pJIT163 using SalI and BamHI restriction sites. I am getting...
25 February 2021 3,221 7 View
I'm currently working having problem with the concentration for recombinant protein use in western blot, the one been used is Recombinant Human CD19 Fc Chimera Protein-rndsystem which does not...
24 February 2021 8,514 3 View
Hello, I am currently using a Cas9 knock in system that will be expressed in the liver. To do so STOP codon fl/fl Cas9 KI mice were bred with Albumin-Cre mice to generate heterozgous pups, that...
24 February 2021 5,759 3 View
Increasingly, more articles are being retracted because of duplicate or otherwise dubious Western blots. Consequently, journals are asking for original Western blots and authentication of...
23 February 2021 8,609 3 View
Hi Everyone, I am a bigginer in western blotting and I am trying to use it for validation of my proteomics data. I have: two regulated proteins with molecular mass of 40 and 73 kDa one control...
23 February 2021 6,671 4 View
I need help using the equation C1V1=C2V2 figuring out how much antibody to add to use for a western blot. The stock was origianlly 3.45 ng/ul but diluted 1:10 so now its 0.345 ng/ul. I need to run...
22 February 2021 9,901 3 View
I run western blot on HCT-116 cell lines, I got actin band like in the attached picture. The band size is not same. Can anyone help me explain this situation and how to overcome? Thank you your...
22 February 2021 6,937 3 View
I ran a western blot using 12% gel and probing for ERK/pERK (cell signaling antibodies). The green is total ERK, the Red is supposed to be phospho-ERK. I see 2 bands below the total ERK that...
22 February 2021 1,777 3 View