How to get good clear slide mount of eriophyid mite collected from a medicinal plant?

Recently I have collected eriophyid mites from leaf erineum of a medicinal plant. When I prepare slide in Hoyer's medium, it is not getting cleared fully, instead I could see fine oil globblets on...

26 September 2020 9,322 5 View

What are the different revese crosslinking protocols?

I need to crosslink my cells for my experiment and at the end, I need to reverse crosslink and extract the proteins. Can anyone tell me what protocols are there for reverse crosslinking after...

23 July 2020 6,257 3 View

I am working in the area of bidirectional DC DC Converters .I want to design a isolated multi port converter for HESS..Can someone help me ?

I am trying to simulate the folowing atached paper"Multiport Isolated Bidirectional DC-DC Converter Interfacing Battery and Supercapacitor For Hybrid Energy Storage Application",by K.Shreelekha...

24 March 2020 2,995 1 View

Energ=y management using bidrectional dc dc converter in electric vehicle?

Could you please help get some literature or MATLAB ,simulations on this topic 'Energy management of electric vehicle using Bidirectional Dc Dc Converter" .. esp wrt 1.recovery of energy in...

29 July 2019 8,494 4 View

Can anyone suggest suppliers for Alumina honeycomb materials?

I require Alumina honeycomb materials with specofic wall thickness, but I struggle to find the place that sells in India.

23 March 2019 736 3 View

Sir? please tell , is possible to apply GA pso in lcl filters in grid connected system please tell ?

sir please tell is possible to apply pso method in filters LCL. please i am asking many numbers , they are telling its not possible in LCL filters Optimization, please is it possible, please tell sir

22 June 2018 5,402 2 View

Why maximum and minimum percentages of steels are mentioned in the IS code for structural elements?

why maximum and minimum percentages of steels are mentioned in the IS code for structural elements?

26 September 2017 7,395 2 View

Can any one explain the relation between the thickness and responsivity of the photodetector ?

09 September 2016 6,919 3 View

What are the different factors effecting the transient stability problems in microgrids?

What are the different factors effecting the transient stability problems in microgrids?

20 April 2016 1,202 3 View

How can we enhance the transient stability of microgrids?

18 April 2016 5,940 5 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Can Ethovision store the generated data and video recordings directly to NAS system?

The size of data generated by Ethovision is huge. Having NAS system for data storage seems to be a good option, I am wondering whether Ethovision can directly store the data to the NAS system...

27 February 2021 6,350 2 View

How can I stain the somatodendritic part of inhibitory neurons in a hippocampal culture with high specificity?

I would like to distinguish the somatodendritic part of the inhibitory neurons in a hippocampal culture (where we expect to have 5-10% of GABAergic neurons). What are the best antibodies and...

23 February 2021 6,756 1 View

Looking for advices and tips to improve the staining and the gating strategy in human blood?

Hi, I am staining a human blood with CD19, CD3,CD14 and CD16. My gating strategy in FlowJo goes like this: - First, I gate a general population SSC-A VS FSC-A => Then gate for single cells, =>...

21 February 2021 5,724 4 View

The PC12 cell line characteristics?

Hello dear scientists, Does the undifferentiated PC12 cell line in terms of chromosome characteristics, proliferation, and drug reactions have the characteristics of cancer cells? Where is it...

21 February 2021 8,945 3 View

What are alternatives for B27+ Supplement in neuronal cell culture?

Hello everybody In our iPSC-derived neuronal culture we use Maturationmedium, supplemented with B27+. As Thermo Fisher run out of stock and can deliver earliest in May, I wanted to ask whether...

16 February 2021 3,348 1 View

How to reduce debris with neuron isolation?

Hi there, I'm isolating neurons from PND1 guinea pigs and have noticed a bit of debris in the early days (this is day 4 plated) and a lot of variability between plates. Current protocol is...

14 February 2021 3,692 4 View

Does anyone have a stack of images of action potentials or abnormal brain scans?

I need a stack of black and white .jpeg/.tiff/.jpg/.png images across time of either action potentials, neurons firing, or brain scans (comparing disease and normal brain, disease progression,...

09 February 2021 2,648 3 View

Help needed: How to map AA, AG, and GG genotypes on to polymorphism information?

Dear Colleagues, I am new to genes and genotyping and I need help. I am trying to figure our how AA, AG, and GG genotypes map on to A1 for DRD2/ANKK1 Taq1A polymorphism(rs1800497) and Val or MET...

02 February 2021 3,861 3 View

Do head direction cells necessarily encode in an allocentric reference frame?

According to the vertebrate literature, I got the impression that head-direction cells are always anchored to an allocentric reference frame. This can easily be tested with freely moving animals....

27 January 2021 3,821 3 View