Such as methanolic extract
Hexane Extract
Water abstraction
Is there a relationship between it and the column used in GC-MS?
And what is the relationship؟
It has shown a difference in the types of compounds identified, based on analysis by GCMS, depending on how the plant extract has been prepared. Probably, many factors are responsible, as you mentioned, the column, the conditions under which it is performed, and so on.
Through the fact that many factors that can affect the presence or disappearance and the lack or increase the proportion of vehicles shown by the analysis, including environmental factors such as temperature and cold, in addition to irrigation and distance between plants
But I did not have a source to talk about the relationship between the type of extractor and column, if you have a source talking about the subject Can you send it to me, thank you for this
Yes. Non-polar solvents like hexane extract non-polar compounds, polar solvents like methanol extract polar compounds (and some inorganics). In general you would analyse non-polar extracts on a non-polar column (e.g. methyl or phenyl-methylsilicone) and polar extracts on a polar column (e.g. polyethyleneglycol wax), mainly to avoid peak distortion from concentration overloading.
During the search for the answer found many factors that affect the quality of the resulting compounds, including the type of plant, including genetic, including environmental factors in addition to the type of column used GC-MS
On the basis of some experiments carried out in our lab for a student dissertation some years ago I would say both the solvents used and the exact method will affect the amount and exact composition of the extract. From personal experience I know that the GC-MS column used will also affect the final results, particularly if the sample contains a wide range of different compounds. To avoid the latter problem you can fractionate your sample before analysis and then analyse the different fractions using different columns and temperature programmes.
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