oh...dear Magdalena...

Yes:

#< Chloroform has an exposure limit of 2 ppm. It affects the central nervous system, heart, liver and kidneys and is classified as "Carcinogen Category 3" (limited evidence of a carcinogenic effect).

Chloroform should only be used in histology if a less toxic substitute cannot be found, and it must be used in a fume cupboard or kept in closed containers.>#

Would you be so kind to define whether you used chloroform as "clearing agent" or as chemical in any processing step before/prior to embedding (into paraffin or celloidine, I guess)...??

Since you questioned expicitely I am not thinking of your using Chloroform for any kind of fixative.

There for sure are some possibilities for substitution but these can be discussed or even pointed to when one knows a little bit more than what you provided with your question (i. e. sort of specimens, at least short description what you have done hitherto, embedding substrate, etc.)

regards and

a beautiful weekend in Innsbruck,

Wolfgang