I'm working on different SERS substrates testing E.coli samples, however, whenever I try to wash the E.coli from the substrate surface the AgNPs layer washes away.
I already use Ethylene glycol during fabrication to preserve the AgNPs on the surface, but I can't keep the AgNPs after washing.
If anyone tried any method that might help I'll be glad if she/he can advise.
Thanks for taking the time