I'm working on different SERS substrates testing E.coli samples, however, whenever I try to wash the E.coli from the substrate surface the AgNPs layer washes away.

I already use Ethylene glycol during fabrication to preserve the AgNPs on the surface, but I can't keep the AgNPs after washing.

If anyone tried any method that might help I'll be glad if she/he can advise.

Thanks for taking the time

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