I want to measure the dry weight of a thiobacillus species which is neutrophil.So I need to separate the produced sulfur before measuring dry weight.
Hello Sir
The problem with most of the solvents that I knew is damaging cell structure,These Solvent damage the lipids of the cell wall and it cause cell disruption which in this case is not favorable as we need undamaged cell structure to measure dry weight
Hi,
If it is just to make the measure why don't just cut your sample in two, one for sulfur isolation the other to measure dry weight?
Antoine
Hi Antonie
Sulfur and Biomass are mixed when I want to measure sulfur I separate biomass by the help of a solvent which is suitable for sulfur but I can not do the same for biomass because most of the solvent damages cell construction
Sepideh
Yes but are you in a preparation setup or analysis? I guess you don't need to recover them after measurement (tell me if I am wrong).
If this is true you can use half of the sample for sulfur measurement (and loose the biomass, and the other half for the biomass measurement.
Antoine
Yes You are right I don't need to recover them after measurement.When I measure Sulfur I do not have any problems because I use Acetone as the solvent and it damages the cell structure but in this case It's not important but When I want to measure the exact dry weight I need to get rid of Sulfur because I do not Want it to be measured in the final weight.The problem Is not solved by dividing the sample into two parts.I look for a way to separate sulfur without damaging the cell so that the cell weight could be measured precisely.
Thank you for taking time to respond me
Sepideh
Hi Ana
As Timothy pointed centrifugation seems to be the only way.By low speed centrifugation you can separate sulfur granules. In my case centrifugation did not help actually as the amount produced sulfur was too low to I had to ignore it and I measured dry weight with sulfur. The important point is that even centrifugatiion can't separate all sulfur content. So I suggest after trying different speed for centrifugation analyze the amount of sulfur ( For example by Sorbo method) and see if it is been separated well enough.
Hope this help
Sepideh
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