Is there a way to prove a relationship between a spinal surgery, a body cast and TLSO, narcotic use, weight gain and apnea? or Type 2 Diabetes?

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Is there any unpublished/published or ongoing works regarding secondary stroke prevention for young poeple living with stroke/TIA and their families?

I am working on a PhD proposal, which I hope to start in October 2024, focusing on non-surgical and non-pharmacological secondary prevention for young people living with stroke/TIA (YPLwS/T)...

23 March 2024 2,505 0 View

Resuspending large bacterial pellets using blender?

I do an 8L induction at 18C for 16-20 hours in TB media. I routinely harvest ~50-80g of bacteria. I freeze my pellets at -20 for > 3 hours after harvesting, and have been using a 1kW blender to...

04 September 2023 1,047 3 View

How to calculate effect sizes from multiple odds ratios for a single exposure variable for a meta-analysis?

For a meta-analysis, I'm including some studies that report the exposure variable in hours as categorical variables (e.g., "1 to 60 mins"; "61 mins to 120 mins"; "121 mins to 180 mins", etc.),...

19 April 2023 6,052 2 View

What are your recommendations in optimizing p53 IHC using Leica Biosystems for the detection of TP53 mutation in endometrial carcinomas?

I will be conducting a study on p53 IHC optimization for Endometrial Ca with subsequent TP53 mutational analysis here in our institution. We will be using RTU antibody (DO-7) but with Leica...

02 February 2023 9,940 0 View

What Universities in the USA have the capability for primary culture of pituitary and thyroid from rats and humans?

I'm looking for a USA-based University that is able to culture rat and human pituitary and thyroid glands to evaluate effects of chemicals on TSH and T3/T4 production. It would be beneficial if...

24 January 2023 8,488 0 View

Recommendation for resources in anthropology (and otherwise) on the changing nature of public space?

As tourism grows and tourists come to dominate public spaces, the value/use of those spaces to native-born populations is shifting. This is a rather new topic for me, but looking for resources...

24 October 2022 1,599 2 View

I am developing a cell and molecular biology laboratory class for upper level undergraduates. What lab manuals do you recommend (or not recommend)?

I would prefer one that is relatively recent and includes a section on gene editing i.e. CRISPR. Thanks much!

20 October 2022 2,589 0 View

What could be a role of System-of-Systems (SoS) in multicellular biology?

How are trillions of cells in a maturing biologic entity coordinated so that the entity can act as a singular "whole" in order to survive?

21 June 2022 3,821 0 View

Can Phosphorus content in plants not reduce under drought stress?

My research investigated the impact of water stress on plant nutrition. Phosphorus content was stable regardless of increasing PEG concentration. Is there any valid explanation on why as i am...

07 June 2022 4,811 7 View

Is there a method of competing risk analysis that does not assume proportional hazards?

The cause-specific hazard model and the Fine-Gray subdistribution model are well known methods of competing risk analysis, but both assumes proportional hazards. Is there any method of handling...

02 June 2022 4,434 1 View

Are there instances where molecules with larger molecular weights exhibit greater mobility than those with smaller molecular weights?

Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...

06 August 2024 1,495 2 View

For an in-vitro drug release study, what molecular weight cut-off (MWCO) dialysis bag is required for a 117 kDa protein?

kindly reply me. Thanking you in advance.

05 August 2024 7,727 4 View

Culture fibroblasts with or without FGF2?

I'm new to primary human fibroblast culture and I'm studying aging using cells from old and young donors. Some research groups have used media supplemented with FGF2, so I cultured cells in the...

29 July 2024 3,030 2 View

Medical question: Is OSA the cause of Afib and HTN?

Does Obstructive Sleep Apnea either cause or exacerbate Atrial fibrillation? Hypertension? We know they are comorbid factors in 50-70% of all OSA patients, and with OSA being so hard to detect...

29 July 2024 7,760 1 View

Is there a good "no frills" search tool for highlighted text?

I am looking for a no frills search tool that will find highlighted text in pdf and epub files. I just want something that will just take me to the next highlighted word or phrase in the way a...

07 July 2024 190 5 View

How is artificial intelligence being utilized to enhance the diagnosis and treatment of sleep apnea?

AI has the potential to improve the management of sleep apnea by personalizing treatment, enhancing diagnostic accuracy, and advancing our understanding of the condition.

03 July 2024 9,393 2 View

Would it be possible to determine how many Cys I have based on Ellman's assay?

I want to predict the DAR ratio based on the number of cys available for conjugation to Cys residue. I use Ellman's assay to predict the concentration of Cys after TCEP reduction. I gain a...

02 July 2024 612 6 View

How to increase molecular weight via polycondensation?

I am working on polymerizing monomers similar to lactic acid, each containing one carboxylic acid and one alcohol group, through a polycondensation technique. Despite using vacuum conditions...

20 June 2024 2,966 4 View

Does anybody know of a cell type with enriched expression of CD63, CD9 and CD81?

I need an appropriate positive control for WB antibodies I am using to confirm the presence of exosomes in samples. The thing is I am having reproducibility issues, and I would like distinguish...

19 June 2024 4,210 3 View

How to find the molecular weight of carbon-based nanoparticles?

I wish to know more on this. Any help or suggestion are highly appreciated. Is there any technique to find it out? Being new in this field.

19 June 2024 3,824 4 View