I'm very much aware that the power output of CO2 laser can be varied by pulse width modulation (PWM). In my case I don't want to alter the laser pulse width but be able to vary the pulse energy. Is there a way around this? Thanks.
That is simple question - use any type of attenuators and all be OK
Does anyone know of any published articles that provide guidance on using TA with multiple team members? I am aware of one (see below), however, there are some disagreements in terms of some of...
23 July 2024 7,819 5 View
I am currently using transwell inserts with the addition of 50 uL of matrigel for an invasion assay with various osteosarcoma cell lines. I have tried various dilutions of matrigel and all result...
17 June 2024 8,695 0 View
Hello, I would really appreciate some help interpreting the output from my simple mediation analysis using PROCESS macro in SPSS please. For context, the X predictor is severity of nausea and...
13 June 2024 9,597 3 View
Hi! I am trying to do RNA extraction from p7 mice's somatosensory cortex and hippocampus. But I am not sure if the RNA was extracted from the correct brain region. I was wondering if there are any...
25 February 2024 5,484 1 View
Hi everyone, I transfected HEK cells with a protein that is intracellularly expressed through flow. Then I isolated the protein using RIPA buffer protocol, however, I accident added IP buffer...
15 February 2024 8,609 3 View
I need to perform a transwell assay on murine BMDMs (bone marrow derived macrophages). I am not sure how long I need to leave the macrophages in incubator to allow the migration. I am finding in...
30 January 2024 2,959 0 View
I already published an experimental research paper entitled "Neuroanthropology: Olfactory Recognition of the Self / Non-self by the Ancestral MHC: An EEG Study" in 2012 (doi:10.5539/ijb.v4n4p1)....
07 January 2024 2,508 4 View
I came across a paper on the exposure of zebrafish to BPA at realistic environmental concentrations and I found no mention of any solvent used to dissolve the BPA. Instead, the exposure was...
09 November 2023 732 1 View
Below is a picture of a 1% gel of my qPCR products from salt marsh sediments (I know, its not the best picture). Standard and NTC are also labeled. I am wondering if my bands are too blurry to be...
12 October 2023 2,739 0 View
I'm doing patch clamp recording in Substantia Nigra pars reticulata(SNr), while I put ChR2 in D1-MSN neurons. During light stimulation, my elicited IPSCs has weird shape of IPSCs. How should I...
29 August 2023 3,107 10 View
i am unable to interpret why its increases in start as shown in figure
11 August 2024 2,179 1 View
I am using Rhodamine6G as gain medium and silver nanoparticles as scatterers on a microscope slide and laser input 532 nm comes from above.
09 August 2024 9,894 2 View
I am interested to know the behavior of dyes toward light. Specifically, Blue dyes re-emit the spectrum, especially from the green zone (known as principal in LED lamps, and blue dyes are known...
05 August 2024 3,290 1 View
I am using a Bruker 600M solid-state NMR spectrometer with a Micro 2.5 microimaging system. The test sample is a tube of 1M LiCl aqueous solution, and the nucleus detected is 1H. I am trying to...
01 August 2024 9,227 1 View
There are three operating parameters - the laser power, the diameter of the focus spot and the scanning speed. It is necessary to obtain a seam of a given depth without pores. What parameter...
27 July 2024 7,168 2 View
Though much literature suggests fitting a Voigt profile on a LIBS peak, is it also possible to fit a Gaussian profile on the LIBS peak? I have 204 spectra files, and I am building a pipeline for...
23 July 2024 6,127 1 View
Hello!!! I want to implement the Swerling characteristics functions (CF) directly in MATLAB without using its Fourier integral pairs...the Swerling CFs are actually Laplace Transform of the signal...
23 July 2024 4,925 1 View
I'm currently working on the implementation of pulses (legumes) in bakery applications. In one case, pulse flours could be added to bread to increase the nutritional value. Pulses (in particular...
17 July 2024 7,293 3 View
I would like to use biofilmQ to analyse confocal laser image, how can do?
16 July 2024 7,628 2 View