I have data from DSC analysis ans would like to plot this on ORIGIN…is there a function to show the thermal denaturation temperature using Origin Software?
Dear Vusi Mshayisa ,
Origin is the data analysis and graphing software. It allows you to create templates for repetitive tasks or even perform batch operations from the user interface without programming. There are several routines already pre-loaded, such as statistics and data handling. Nevertheless, you need to load your DSC data and work on it to extract the necessary data.
If someone has any routine specifically written or set up to find the denaturation temperature, it would be personal batch programming.
There are integration and derivation functions that you may use for your purpose.
Best regards
WNM
Dear all, the attached paper gives a comparison of different software s processing of denaturation data. My Regards
This is another interesting document.
https://doi.org/10.1016/S0168-1656(00)00243-1
Thanks Wagner Da Nova Mussel and Abdelkader BOUAZIZ what I was looking for is the steps to follow to analyse this data...I will continue searching and reading...
Hi ;
In the case of the OriginPro, the temperature and enthalpy of denaturation can be determined by deconvolution of the peaks using Gaussian functions. The Origin PeakFit module can be used for deconvolution of DSC thermograms. The protein denaturation temperature is determined from the maximum peak temperature and the enthalpy of denaturation is calculated as the area under the endothermic curve. It is also possible to use Pyris software to study DSC thermograms of protein denaturation.
With my best regards
Dear Vusi Mshayisa;
Please check the following documents.
With my best regards
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