Is anyone can help me about cutting pET32a expression vector?

I am going to clone a gene into pET32 a expression vector. After completing the cloning process, I verified my insertion by PCR an I see about 800 bp product . Then also, I wanted to verify with...

12 October 2020 6,925 2 View

Do you think about scientific activities are the subject of capitalism itself?

Do you think it is true, to see a scientific study as a source of income, from the race to be published by the journals to the publication of the symposium? Yes, money is spent on scientific...

01 January 1970 2,975 4 View

Cutting pET32a expression vector

I am going to clone a gene into pET32 a expression vector. After completing the cloning process, I verified my insertion by PCR an I see about 800 bp product . Then also, I wanted to verify with...

01 January 1970 1,223 6 View

What determines, reveals and directs human behavior?

What determines human behavior in daily life?

01 January 1970 155 27 View

What should be the criterion for the advancement of an academic career?

What should be the most important criteria for appointment to the career steps in universities? What are the conditions deserve to progress on the career steps? What are the conditions of...

01 January 1970 4,899 3 View

Is an idea everything which passes one's through our minds?

How to define an idea. What is that criteria?

01 January 1970 9,116 10 View

Dear all, I was wondering if anyone knows whether MGLtools and Autodock can run on Mac OS?

I have tried to download the 64-bit version of MGLtools however, as many times as I have downloaded and uninstall the programs, an error arises that the app needs to be updated. Thank you.

03 March 2021 5,997 2 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10?

What's the best way to measure growth rates in House sparrow chicks from day 2 to day 10? Since, the growth curve from day 2 to 10 won't be like the "Logistic curve" it might not follow logistic...

03 March 2021 1,401 3 View

I got 02 moderator values defining Johnson-Neyman significance regions. Is it an error?

Hi everyone, I am conducting research for my Master's thesis. I am using PROCESS by Johnson-Neyman to analyze my Moderator model. I test the relationship between Public Service Motivation and...

03 March 2021 2,350 2 View

How to access MSCI KLD Stats and Sustainalytics?

Hello all, I'm currently working on my undergraduate thesis and I'm having difficulties trying to access KLD Stats and Sustainalytics because my campus Airlangga University and apparently other...

02 March 2021 8,525 1 View

Do I need to do linear regression before running mediation analysis using PROCESS MACRO by Hayes ?

I just wanted to check if I need to run a linear regression separately if I am using PROCESS MACRO to run mediation analysis. Thank you.

02 March 2021 4,359 3 View

Ideas for Phd's Thesis on pricing (Marketing)?

Hi everyone, I'm studying Marketing and I would like to write my PhD thesis on the topic of pricing. Any specific ideas?

02 March 2021 9,706 5 View

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02 March 2021 3,060 3 View

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02 March 2021 932 1 View

Do I need to strip the PVDF membrane before staining it with colloidal gold?

Dear Colleagues, After running Western blot on PVDF membrane and detection using ECL, I would like to stain my PVDF with colloidal gold to be able to allign the ECL image with total proteins on...

02 March 2021 7,829 3 View