Does anyone know of a commercially available antibody that specifically recognizes VEGFA isoform 145? Any recommendations or experience with such an antibody would be greatly appreciated.
Regarding your question about a VEGFA145 isoform-specific antibody, to my knowledge, commercially available antibodies that exclusively recognize the VEGFA145 isoform are quite rare. Most VEGFA antibodies target common epitopes shared among the major isoforms (e.g., VEGFA121, VEGFA165, VEGFA189).
I recommend checking major antibody suppliers such as Abcam, Thermo Fisher, or Sigma-Aldrich for any updated products. Additionally, contacting the technical support teams of these companies might help clarify antibody specificity.
If no isoform-specific antibody is available, alternative approaches include designing custom antibodies targeting unique peptide sequences of VEGFA145 or using isoform-specific qPCR or RNA-based methods to differentiate isoform expression.
If you need assistance finding literature on VEGFA isoforms or designing validation experiments, feel free to ask