I and my collegues research radiation resistance in bacteria in the University of Isfahan and we need the standard strain of Deinococcus radiodurans for confirmtion of our results.
http://www.atcc.org/ATCCAdvancedCatalogSearch/ProductDetails/tabid/452/Default.aspx?ATCCNum=13939&Template=bacteria
I would check (and compare) those gene encoding for DNA repair (rec operon and similar).
thank you for response to me but because international boycott I dont buy this strain.
can you help me?
If you are going to work at the molecular genetics level, ask for purified DNA to a laboratory growing this bacterium. This is almost costless and shipping is easy since no living samples are involved.
I suggest pprA and pprI (irrE) genes specific for radioresistance of Deinococcus species.
Stander strain, you can obtained from DSMZ (Germany) or ATTC(USA)
i work on radiation and it effect on bacteria , but Deinococcus species not my target
any way you need isolate DNA in control stage without radiation and isolate DNA at radiation dose you need and try make partial sequence to compare
or study determin gene in your bactera after and before radiated
thanks for your response me, but I live in iran and because boycott I cant buy this strain
my thesis is regarding isolation radiation resistance bacteria but I have not standard strain can you help me?
If culture collections refuse to send you a strain (have you really approached all of them?), then you could find out who isolated the strains and contact those microbiologists. But I would first try contacting culture collections. There are culture collections all over the world. Here is a list: http://wdcm.nig.ac.jp/hpcc.html
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