Is there a simple and rapid quantitative assay for phytosterols in a plant extract?

More Nícolas de Castro Campos Pinto's questions See All

Is there any solvent that may replace Cryocool in SpeedVac Thermo Fisher system?

We have the Thermo Fisher SpeedVac Savant SPD1010 system in our laboratory. The manufacturer recommends the use of Cryocool as heat transfer fluid to conducts heat away from the glass condensation...

02 March 2019 6,637 2 View

Strugglling with m6A dot blot any suugesstion ?

I have been doing the m6A dot blot for a while with no improvement, I am extracting the RNA, and I can see the dots although the three biological replicas give a different reading on the memberan...

10 August 2024 8,539 5 View

How can I use the cif data obtained from rietveld refinement extracted via gsas2, for microstructural analysis using ETEX software?

09 August 2024 7,718 0 View

How do soil microflora interact with plant roots and influence plant nutrition, health, and productivity?

06 August 2024 9,618 3 View

The aqueous fraction of the hydroethanolic extract is showing the presence of palmitic acid. What is the mechanism responsible ?

Palmitic acid presence in aqueous fraction

05 August 2024 8,624 4 View

Which solvent is better to dissolve with secondary metabolites extracted from fungi?

I work on MCF7 cell cell for anticaner purpose and I wa to do drug preperation the drug ( secondary metabolites extracted from Aspergillus) My question which solvent is better with these secodary...

03 August 2024 4,725 2 View

For systematic review data extraction, should I use ITT or PP analysis, and count all randomized participants or only those who completed the study?

I am conducting a systematic review and meta analysis; as I am extracting data I realized there is a consort diagram that shows the number of patients from randomization till end of the study. So...

01 August 2024 9,993 3 View

For the moringa oleifera extract using ethanol as the solvent,what is the alternative method to concentrate the extract other than rotary evaporation?

99% pure ethanol was used for maceration also alternate methods along with the temperature and time to concentrate the extract can be specified

31 July 2024 5,113 4 View

How do soil microbes affect plant health and productivity and how do plants interact with soil microorganisms and contribute to soil fertility?

31 July 2024 5,087 4 View

What is the role of decomposers in the cycling of matter in the biosphere and role do soil microbes play in plant nutrition availability and uptake?

31 July 2024 7,921 5 View

What are the factors that causes the sample to have higher efficacy at lower concentration?

We conducted an antibacterial study of a plant extract. Varying concentrations of crude extract were subjected to microbroth dilution assay. The result showed that only the lowest concentration of...

31 July 2024 8,666 3 View

Niko Radulović

Dear Nicolas,

I came across a paper that might be helpful to you (attached to this message).

If it does not suit your needs, just say and I will try to find something else.

All the best,

Niko

Nícolas de Castro Campos Pinto

Dear Niko,

This paper was really helpful. Thank you very much.

Bapuji Maringanti

Dr.Niko and Nicolas: I feel this technique could be straight applied on TLC plate and using a densitometer/scanner, you can make a rapid estimation of total and individual phytosterols. pl try.