I'm currently using RStudio to analyze qPCR results from an experiment. I was using the pcr package (v. 1.2.2) to perform a delta-delta Ct analysis and compare the results. However, I want to account for varying efficiency values between genes which requires the Pfaffl (Standard-Curve) method. While the math for this method is straight-forward, the error propagation (and accounting for the efficiency error) is fairly involved so I would like to use a well-validated package to do this. Does anyone know of any R Studio packages that accommodates the Pfaffl method to perform qPCR analysis? I have not been able to find any free software for this aside from REST, but REST doesn't work on any modern OS.
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