How to convert 2D stack images to 3D to obtain surface area of nucleus?

I have 15 slices of the nucleus at different z-heights from confocal imaging. I would like to use these slices to reconstruct the 3D nucleus and ultimately estimate the surface area of the...

20 February 2021 6,967 1 View

I want to convert Methane gas to liquid Methanol using photocatalysis. Can you please suggest me a good paper which I can study ?

There are a number of publications regarding this, but none of them are clear and show the mechanism of gaseous Methane conversion to liquid Methanol.

06 January 2021 364 3 View

Can wavelet packet sub-bands be stacked together to form an image?

I have a 1D signal and I have done wavelet packet decomposition on it which is giving several sub-bands. Can I stack these sub-bands (one below other) to form a 2D matrix and hence an image...

27 November 2020 1,269 5 View

How can I maintain a surface at isothermal condition, say at 300 deg Celsius.?

I have 6 heaters each of power 0.5 kW and mounted below a curved mild steel sheet of thickness 4 mm. The heaters and the below section is covered and insulated. I want the top surface to be...

05 November 2020 199 5 View

what are the possible reasons for Crop Water Stress Index values higher than 1 under moisture stress condition?

I have determined CWSI using the theoretical approach of Jackson et al., (1982) based on canopy temperature measurement in chickpea. at the final stages of crop growth some of my values of CWSI...

28 October 2020 9,992 3 View

Do always a Good paper depends on How much Citations it Received. I have seen many Good Research Papers with zero or less Than 10 Citations?

Its a Research Paper related Question

22 October 2020 6,179 4 View

Help in cloning, should I create 5' phosphate group on digested vector?

Hello all, I am trying to introduce an annealed DNA fragment into the double digested vector. The ssDNAs were ordered without a phosphate group modification. The vector is digested with XhoI and...

20 October 2020 8,357 5 View

Can anyone explain the calculation process of integrated biomarker response (IBR) used in chronic toxicity study with an arbitrary dataset?

Video tutorial on IBR calculation or stepwise procedure..

05 October 2020 9,279 3 View

Could someone provide the permeability value for 2% Chromium doped BiFeO3?

Any close value will also work.

03 October 2020 8,067 4 View

Purification of annealed oligonucleotides after annealing procedure?

Hello all, I will do oligonucleotides annealing for the first time which will be later used for cloning without any more modification. Can any of you kindly suggest if I should purify the annealed...

16 September 2020 3,881 3 View

What is the status of bread making and more bakery foods with the use of insect flour/powder?

Insects have been consumed by human diet since prehistoric times. At present, the idea of looking at insects as food has been getting traction worldwide, also in response to increasing food...

03 March 2021 6,223 5 View

How to grow E.coli on the low salt LB agar plate with 50 or 100ug/ml of Blasticidin?

Hello, I would like to ask how to grow E.coli which resists 50 or 100ug/ml of Blasticidin. I have tried many methods by using low salt Luria-Bertain(LB) agar plate. However, no colonies grow. Let...

01 March 2021 2,027 2 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

How to calculate the crystallinity of cellulose film?

27 February 2021 2,753 4 View

Why initial concentration of Pb not the same as calculated one when testing using AAS?

I am currently doing research on the adsorption of heavy metal using magnetic reduce graphene oxide. I use AAS to determine the concentration of the heavy metal. I prepare the Pb solution with...

26 February 2021 9,580 4 View

Is this the write procedure for acid digestion of soil for heavy metal analysis using AAS?

How to do acid digestion of soil sample with 3:1 aquaregia in an open vessel?

23 February 2021 3,053 7 View

Can a semi-conductor material have different absorption coefficient?

Does the absorption coefficient of the semiconductor changes with the change in the structure of the semiconductor? If you have a thin film and nanowire of the same semiconductor, will the...

23 February 2021 9,799 4 View

Have anyone checked before Cyclic voltammograms of intial ITO substrate and E-teck?

As a beginner in electrochemistry, I tried to obtain cyclic voltamograms of commercial anatase powder deposited on an ITO substrate or on Eteck. After several attempts, I noticed that my cyclic...

23 February 2021 3,175 3 View

How to reduce static electricity of powder after lyophilization?

I am working on synthesizing and then purifying peptides using the SPPS system. After purification of the peptide, the peptide powder is obtained using a freeze dryer. When measuring the powder,...

22 February 2021 10,099 5 View

What kind of steel moulds are used for hot pressing metal powders?

My main concern is that the metal powders should not weld to the mold surface and I should easily be able to eject out the pressed samples. I am looking for alternative for graphite moulds. The...

22 February 2021 2,965 4 View