the results I got are oppose to each others ... hydrogen peroxide scavenging decreased and the inhibition activity of DPPH increased .. Is there a relationship between these methods?
OH and DPPH radical scavenging assays are electron transfer (ET) based assays.
There should not be any negative correlation b/w them.
Try standardizing your protocol and perform multiple replicates.
What are your reaction conditions ? Often hydroperoxide scavenging assays are done in a more lipophilic medium. Howver what do you expect during the hydroperoxide assay: Either scavenging hydroxyl radicals, or peroxyl radicals, or (in some assays) Lipid = alkyl radicals....from the chemical structure they´are not even close to the structure of DPPH...further you have not said a word about your scavenging samples. can be also that some radicals are sterically hindered while others are not....and all of the mentioned radicals have quite short half-life times compared to DPPH. So, it seems to be obvious that there might be differences. This is what studying antioxidant activity is about: "Expect the unexpected" !!!
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