Ferdinand Kirchner

3 Questions 4 Answers 0 Followers

Questions related from Ferdinand Kirchner

Using more than three degenerated codons simultaneously?

I want to perform iterative saturation mutagenesis. I did a cast analysis of my protein of interest (10A). As a result i have ~75 AA positions since I have a very limited time frame (5 months). I...

09 October 2013 8,655 4 View

How do I set up different gas concentrations in a sealed tube?

I'm going to work with methanotrophs in my master thesis. Since I'm the first one to work with methane in our group we are not sure how to set up different concentrations like 10% or 20% methane...

26 April 2013 9,486 0 View

Is there a protocol for the isolation of free fatty acids and fatty acyl-CoA/ACP (prokaryotes)? And dectection with GC-MS?

I want to use free fatty acids or the thioesters for a pathway and want to see if I can detect the them in the wildtype. The protocols I find extract the fatty acids from phospholipids if I...

25 February 2013 9,857 0 View