You might want to look at isoelectric focussing where the proteins move to their isoelectric point and then stop moving. Good for separation and also for concentrating the individual proteins

There is no special protocol for serum proteins SDS-PAGE. SDS-PAGE is performed similarly with all samples but may involve slight modifications. If you can shed more light on your requirements maybe I'll be able to help you.

We intend to identify albumin, α1, α2, β  and γ fraction from serum in order to determine their %

Hi Miriam, there are a number of methods that you can use to enrich for serum albumin:

https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1243434/?page=1

https://www.ncbi.nlm.nih.gov/pubmed/462920

https://www.researchgate.net/publication/286586254_A_new_protocol_for_purifying_human_serum_albumin

http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000800002

https://www.linkedin.com/pulse/isolation-albumin-from-horse-serum-using-affinity-nichole-mcghie

Article A new protocol for purifying human serum albumin