LigaTrap's Human IgA purification column and Cytiva's HiTrap Protein A column do not isolate IgA type 1 and 2 from other immunoglobulins. LigaTrap's technical service informed me that their resin would bind other immunoglobulins, not just IgAs. I tried Cytiva's column with the understanding that it would capture IgA in the column, but it did not. What we are setting out to do is very simple, we want to deplete a commercial CSF of IgAs, so we need a column that will bind IgAs only. I have not been successful in finding a product that does this, and would appreciate any help or advice. Thank you!
CaptureSelect™ IgA-XL Affinity Matrix
Catalog number: 2943972005
I have not used this particular affinity resin from Thermo Fisher but I have used some of their other resins from this same line. Some are specific, some have non-specific binding of other proteins. You could also try coupling an IgA antibody (alpha chain only) to CNBr Sepharose. Just make sure that the antibody does not cross react to other heavy chains or to light chains (Kappa and Lambda).
Hope this helps!
- Badges
- Science topic
- Similar topics
- Chemistry
- Electrochemistry